Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF879 and GFF880 are separated by 226 nucleotides GFF880 and GFF881 are separated by 174 nucleotides
GFF879 - Inorganic pyrophosphatase (EC 3.6.1.1), at 58,090 to 58,620
GFF879
GFF880 - Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11), at 58,847 to 59,845
GFF880
GFF881 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 60,020 to 61,399
GFF881
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1 at 58.748 kb on - strand at 58.827 kb on - strand at 58.841 kb on + strand at 58.849 kb on - strand at 58.958 kb on - strand, within GFF880 at 58.958 kb on - strand, within GFF880 at 58.966 kb on + strand, within GFF880 at 59.077 kb on - strand, within GFF880 at 59.130 kb on + strand, within GFF880 at 59.142 kb on + strand, within GFF880 at 59.217 kb on + strand, within GFF880 at 59.222 kb on - strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.267 kb on - strand, within GFF880 at 59.267 kb on - strand, within GFF880 at 59.307 kb on + strand, within GFF880 at 59.364 kb on + strand, within GFF880 at 59.365 kb on - strand, within GFF880 at 59.374 kb on - strand, within GFF880 at 59.533 kb on - strand, within GFF880 at 59.533 kb on - strand, within GFF880 at 59.603 kb on - strand, within GFF880 at 59.825 kb on - strand at 60.141 kb on + strand at 60.142 kb on - strand at 60.177 kb on - strand, within GFF881 at 60.283 kb on + strand, within GFF881 at 60.283 kb on + strand, within GFF881 at 60.284 kb on - strand, within GFF881 at 60.504 kb on - strand, within GFF881
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 58,748 - +0.1 58,827 - +0.2 58,841 + -0.2 58,849 - -1.1 58,958 - GFF880 0.11 -0.2 58,958 - GFF880 0.11 -0.3 58,966 + GFF880 0.12 -0.4 59,077 - GFF880 0.23 -0.1 59,130 + GFF880 0.28 -0.6 59,142 + GFF880 0.30 -0.4 59,217 + GFF880 0.37 -1.0 59,222 - GFF880 0.38 -0.7 59,266 + GFF880 0.42 +0.3 59,266 + GFF880 0.42 -0.1 59,266 + GFF880 0.42 -0.2 59,267 - GFF880 0.42 -0.5 59,267 - GFF880 0.42 -0.3 59,307 + GFF880 0.46 -1.8 59,364 + GFF880 0.52 -1.3 59,365 - GFF880 0.52 -0.3 59,374 - GFF880 0.53 -0.1 59,533 - GFF880 0.69 +0.3 59,533 - GFF880 0.69 -0.5 59,603 - GFF880 0.76 +0.2 59,825 - -0.3 60,141 + +0.0 60,142 - -0.2 60,177 - GFF881 0.11 -0.3 60,283 + GFF881 0.19 -0.3 60,283 + GFF881 0.19 -0.2 60,284 - GFF881 0.19 -0.1 60,504 - GFF881 0.35 +0.3
Or see this region's nucleotide sequence