Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF760

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF759 and GFF760 are separated by 9 nucleotidesGFF760 and GFF761 overlap by 4 nucleotidesGFF761 and GFF762 overlap by 17 nucleotides GFF759 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 116,649 to 117,782 GFF759 GFF760 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 117,792 to 118,745 GFF760 GFF761 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 118,742 to 119,587 GFF761 GFF762 - Putative inner membrane protein, at 119,571 to 120,134 GFF762 Position (kb) 117 118 119Strain fitness (log2 ratio) -2 -1 0 1at 116.930 kb on + strand, within GFF759at 116.930 kb on + strand, within GFF759at 117.084 kb on - strand, within GFF759at 117.152 kb on + strand, within GFF759at 117.152 kb on + strand, within GFF759at 117.528 kb on + strand, within GFF759at 117.528 kb on + strand, within GFF759at 117.528 kb on + strand, within GFF759at 117.529 kb on - strand, within GFF759at 117.538 kb on + strand, within GFF759at 117.615 kb on - strand, within GFF759at 117.621 kb on + strand, within GFF759at 117.638 kb on + strand, within GFF759at 117.643 kb on - strand, within GFF759at 117.778 kb on + strandat 117.778 kb on + strandat 117.780 kb on + strandat 117.901 kb on - strand, within GFF760at 118.235 kb on - strand, within GFF760at 118.354 kb on - strand, within GFF760at 118.355 kb on + strand, within GFF760at 118.369 kb on - strand, within GFF760at 118.391 kb on - strand, within GFF760at 118.401 kb on + strand, within GFF760at 118.593 kb on - strand, within GFF760at 118.668 kb on - strandat 118.670 kb on + strandat 118.670 kb on + strandat 118.671 kb on - strandat 118.671 kb on - strandat 118.687 kb on + strandat 118.688 kb on - strandat 118.710 kb on - strandat 118.747 kb on + strandat 118.748 kb on - strandat 118.802 kb on - strandat 118.895 kb on + strand, within GFF761at 119.230 kb on - strand, within GFF761at 119.671 kb on + strand, within GFF762

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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116,930 + GFF759 0.25 +0.6
116,930 + GFF759 0.25 +0.3
117,084 - GFF759 0.38 -0.0
117,152 + GFF759 0.44 +0.2
117,152 + GFF759 0.44 +0.2
117,528 + GFF759 0.78 +1.0
117,528 + GFF759 0.78 -1.5
117,528 + GFF759 0.78 +0.1
117,529 - GFF759 0.78 +0.1
117,538 + GFF759 0.78 +0.2
117,615 - GFF759 0.85 -0.1
117,621 + GFF759 0.86 +0.6
117,638 + GFF759 0.87 +0.2
117,643 - GFF759 0.88 +0.1
117,778 + +0.3
117,778 + +0.4
117,780 + +0.2
117,901 - GFF760 0.11 -1.2
118,235 - GFF760 0.46 +0.0
118,354 - GFF760 0.59 -1.8
118,355 + GFF760 0.59 -0.5
118,369 - GFF760 0.60 -1.1
118,391 - GFF760 0.63 -0.1
118,401 + GFF760 0.64 -0.5
118,593 - GFF760 0.84 -0.4
118,668 - -0.3
118,670 + +0.5
118,670 + +0.1
118,671 - -0.7
118,671 - +0.2
118,687 + +0.4
118,688 - -0.3
118,710 - -0.4
118,747 + +0.5
118,748 - +0.3
118,802 - -0.6
118,895 + GFF761 0.18 +0.2
119,230 - GFF761 0.58 +0.2
119,671 + GFF762 0.18 +0.3

Or see this region's nucleotide sequence