Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF722 and GFF723 are separated by 5 nucleotides GFF723 and GFF724 are separated by 277 nucleotides
GFF722 - Pyruvate formate-lyase (EC 2.3.1.54), at 78,411 to 80,843
GFF722
GFF723 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 80,849 to 81,748
GFF723
GFF724 - Molybdopterin biosynthesis protein MoeB, at 82,026 to 82,775
GFF724
Position (kb) 80
81
82 Strain fitness (log2 ratio) -1
0
1 at 79.866 kb on + strand, within GFF722 at 79.866 kb on + strand, within GFF722 at 80.038 kb on + strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.111 kb on + strand, within GFF722 at 80.112 kb on - strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.124 kb on - strand, within GFF722 at 80.435 kb on - strand, within GFF722 at 80.490 kb on - strand, within GFF722 at 80.574 kb on - strand, within GFF722 at 80.651 kb on + strand at 80.652 kb on - strand at 80.725 kb on + strand at 80.737 kb on + strand at 80.778 kb on - strand at 80.847 kb on - strand at 80.847 kb on - strand at 80.890 kb on - strand at 80.909 kb on + strand at 80.946 kb on + strand, within GFF723 at 80.960 kb on + strand, within GFF723 at 80.971 kb on + strand, within GFF723 at 81.226 kb on + strand, within GFF723 at 81.227 kb on - strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.440 kb on + strand, within GFF723 at 81.615 kb on - strand, within GFF723 at 81.692 kb on + strand at 81.693 kb on - strand at 81.727 kb on - strand at 81.857 kb on - strand at 81.892 kb on - strand at 81.969 kb on - strand at 81.979 kb on - strand at 82.006 kb on + strand at 82.099 kb on + strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.149 kb on - strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.330 kb on - strand, within GFF724 at 82.346 kb on + strand, within GFF724 at 82.349 kb on - strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.375 kb on - strand, within GFF724 at 82.434 kb on - strand, within GFF724 at 82.467 kb on - strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.515 kb on + strand, within GFF724 at 82.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 79,866 + GFF722 0.60 -0.1 79,866 + GFF722 0.60 -0.6 80,038 + GFF722 0.67 -0.2 80,039 - GFF722 0.67 +0.1 80,039 - GFF722 0.67 +0.1 80,111 + GFF722 0.70 +0.2 80,112 - GFF722 0.70 -0.3 80,115 + GFF722 0.70 +0.0 80,115 + GFF722 0.70 -0.7 80,115 + GFF722 0.70 +0.6 80,115 + GFF722 0.70 -0.3 80,116 - GFF722 0.70 -1.4 80,116 - GFF722 0.70 -0.2 80,116 - GFF722 0.70 -0.1 80,124 - GFF722 0.70 -0.4 80,435 - GFF722 0.83 -0.1 80,490 - GFF722 0.85 -1.2 80,574 - GFF722 0.89 +0.2 80,651 + -0.0 80,652 - -0.3 80,725 + +0.2 80,737 + +0.5 80,778 - +0.2 80,847 - -1.2 80,847 - +0.8 80,890 - -0.2 80,909 + -0.1 80,946 + GFF723 0.11 +0.4 80,960 + GFF723 0.12 -1.1 80,971 + GFF723 0.14 -0.3 81,226 + GFF723 0.42 +0.1 81,227 - GFF723 0.42 -0.1 81,228 + GFF723 0.42 -0.6 81,228 + GFF723 0.42 -1.1 81,440 + GFF723 0.66 -0.8 81,615 - GFF723 0.85 -0.3 81,692 + +0.1 81,693 - -1.6 81,727 - +0.0 81,857 - -0.4 81,892 - +1.0 81,969 - -0.1 81,979 - +0.0 82,006 + +0.6 82,099 + +0.2 82,100 - +0.1 82,100 - +0.3 82,100 - +0.4 82,149 - GFF724 0.16 +0.3 82,283 + GFF724 0.34 +0.7 82,283 + GFF724 0.34 +0.7 82,330 - GFF724 0.41 +0.6 82,346 + GFF724 0.43 -1.1 82,349 - GFF724 0.43 +1.0 82,374 + GFF724 0.46 +0.8 82,374 + GFF724 0.46 +0.7 82,375 - GFF724 0.47 -0.1 82,434 - GFF724 0.54 -0.6 82,467 - GFF724 0.59 +0.1 82,475 + GFF724 0.60 -0.4 82,475 + GFF724 0.60 +1.0 82,515 + GFF724 0.65 +0.1 82,727 + -1.1
Or see this region's nucleotide sequence