Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF67

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF66 and GFF67 overlap by 1 nucleotidesGFF67 and GFF68 are separated by 26 nucleotides GFF66 - H(+)/Cl(-) exchange transporter ClcA, at 49,007 to 50,428 GFF66 GFF67 - hypothetical protein, at 50,428 to 50,571 GFF67 GFF68 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 50,598 to 51,878 GFF68 Position (kb) 50 51Strain fitness (log2 ratio) -1 0 1at 49.471 kb on + strand, within GFF66at 49.471 kb on + strand, within GFF66at 49.472 kb on - strand, within GFF66at 49.472 kb on - strand, within GFF66at 49.550 kb on - strand, within GFF66at 49.595 kb on - strand, within GFF66at 49.666 kb on - strand, within GFF66at 49.736 kb on + strand, within GFF66at 49.829 kb on - strand, within GFF66at 49.997 kb on + strand, within GFF66at 50.166 kb on - strand, within GFF66at 50.294 kb on - strandat 50.448 kb on - strand, within GFF67at 50.451 kb on - strand, within GFF67at 50.553 kb on + strand, within GFF67

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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49,471 + GFF66 0.33 +0.1
49,471 + GFF66 0.33 +0.1
49,472 - GFF66 0.33 -0.1
49,472 - GFF66 0.33 -0.5
49,550 - GFF66 0.38 +0.1
49,595 - GFF66 0.41 -0.2
49,666 - GFF66 0.46 +0.4
49,736 + GFF66 0.51 +0.1
49,829 - GFF66 0.58 -0.5
49,997 + GFF66 0.70 -0.3
50,166 - GFF66 0.82 -0.7
50,294 - +0.9
50,448 - GFF67 0.14 +0.5
50,451 - GFF67 0.16 -0.0
50,553 + GFF67 0.87 -1.1

Or see this region's nucleotide sequence