Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF629 and GFF630 are separated by 140 nucleotides GFF630 and GFF631 are separated by 97 nucleotides
GFF629 - Shikimate kinase I (EC 2.7.1.71), at 370,370 to 370,855
GFF629
GFF630 - 3-dehydroquinate synthase (EC 4.2.3.4), at 370,996 to 372,000
GFF630
GFF631 - DamX, an inner membrane protein involved in bile resistance, at 372,098 to 373,375
GFF631
Position (kb) 370
371
372
373 Strain fitness (log2 ratio) -2
-1
0
1 at 370.022 kb on + strand at 370.022 kb on + strand at 370.022 kb on + strand at 370.022 kb on + strand at 370.023 kb on - strand at 370.312 kb on + strand at 370.313 kb on - strand at 370.318 kb on + strand at 370.558 kb on + strand, within GFF629 at 370.558 kb on + strand, within GFF629 at 370.811 kb on - strand at 370.837 kb on + strand at 370.867 kb on + strand at 370.890 kb on + strand at 370.890 kb on + strand at 370.890 kb on + strand at 370.891 kb on - strand at 370.891 kb on - strand at 370.923 kb on + strand at 370.944 kb on + strand at 371.137 kb on - strand, within GFF630 at 371.384 kb on - strand, within GFF630 at 371.473 kb on + strand, within GFF630 at 371.481 kb on + strand, within GFF630 at 371.566 kb on - strand, within GFF630 at 371.627 kb on + strand, within GFF630 at 371.636 kb on + strand, within GFF630 at 371.652 kb on + strand, within GFF630 at 371.652 kb on + strand, within GFF630 at 371.652 kb on + strand, within GFF630 at 371.699 kb on + strand, within GFF630 at 371.699 kb on + strand, within GFF630 at 371.725 kb on - strand, within GFF630 at 371.778 kb on + strand, within GFF630 at 371.854 kb on + strand, within GFF630 at 371.855 kb on - strand, within GFF630 at 371.888 kb on + strand, within GFF630 at 372.095 kb on + strand at 372.151 kb on + strand at 372.253 kb on + strand, within GFF631 at 372.262 kb on + strand, within GFF631 at 372.386 kb on + strand, within GFF631 at 372.387 kb on - strand, within GFF631 at 372.877 kb on + strand, within GFF631
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 370,022 + +0.3 370,022 + -0.1 370,022 + +0.5 370,022 + +0.1 370,023 - -0.1 370,312 + +0.2 370,313 - +0.0 370,318 + -0.1 370,558 + GFF629 0.39 -1.3 370,558 + GFF629 0.39 +0.4 370,811 - +0.1 370,837 + -0.5 370,867 + +1.7 370,890 + -0.1 370,890 + -0.2 370,890 + +0.1 370,891 - -0.2 370,891 - -0.4 370,923 + +1.0 370,944 + +0.0 371,137 - GFF630 0.14 -2.0 371,384 - GFF630 0.39 -0.6 371,473 + GFF630 0.47 -1.3 371,481 + GFF630 0.48 -0.9 371,566 - GFF630 0.57 -0.5 371,627 + GFF630 0.63 -0.3 371,636 + GFF630 0.64 +0.2 371,652 + GFF630 0.65 -1.6 371,652 + GFF630 0.65 +0.4 371,652 + GFF630 0.65 +0.7 371,699 + GFF630 0.70 -0.6 371,699 + GFF630 0.70 +0.4 371,725 - GFF630 0.73 -1.2 371,778 + GFF630 0.78 -0.0 371,854 + GFF630 0.85 -0.8 371,855 - GFF630 0.85 -2.2 371,888 + GFF630 0.89 -1.9 372,095 + -0.2 372,151 + +0.4 372,253 + GFF631 0.12 +0.6 372,262 + GFF631 0.13 +0.8 372,386 + GFF631 0.23 +0.1 372,387 - GFF631 0.23 +0.1 372,877 + GFF631 0.61 +0.2
Or see this region's nucleotide sequence