Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4890 and GFF4891 are separated by 0 nucleotides GFF4891 and GFF4892 are separated by 113 nucleotides
GFF4890 - Transport ATP-binding protein CydC, at 5,948 to 7,669
GFF4890
GFF4891 - Transport ATP-binding protein CydD, at 7,670 to 9,436
GFF4891
GFF4892 - Thioredoxin reductase (EC 1.8.1.9), at 9,550 to 10,518
GFF4892
Position (kb)
7
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6.887 kb on + strand, within GFF4890 at 6.887 kb on + strand, within GFF4890 at 6.888 kb on - strand, within GFF4890 at 6.888 kb on - strand, within GFF4890 at 7.204 kb on + strand, within GFF4890 at 7.358 kb on + strand, within GFF4890 at 7.359 kb on - strand, within GFF4890 at 7.377 kb on + strand, within GFF4890 at 7.492 kb on + strand, within GFF4890 at 7.493 kb on - strand, within GFF4890 at 7.495 kb on - strand, within GFF4890 at 7.495 kb on - strand, within GFF4890 at 7.617 kb on - strand at 7.627 kb on + strand at 7.651 kb on - strand at 7.672 kb on - strand at 7.672 kb on - strand at 8.012 kb on + strand, within GFF4891 at 8.108 kb on + strand, within GFF4891 at 8.136 kb on - strand, within GFF4891 at 8.233 kb on - strand, within GFF4891 at 8.249 kb on + strand, within GFF4891 at 8.252 kb on + strand, within GFF4891 at 8.369 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.470 kb on + strand, within GFF4891 at 8.497 kb on + strand, within GFF4891 at 8.506 kb on - strand, within GFF4891 at 8.657 kb on - strand, within GFF4891 at 8.899 kb on + strand, within GFF4891 at 9.027 kb on + strand, within GFF4891 at 9.027 kb on + strand, within GFF4891 at 9.030 kb on + strand, within GFF4891 at 9.030 kb on + strand, within GFF4891 at 9.040 kb on + strand, within GFF4891 at 9.041 kb on - strand, within GFF4891 at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.284 kb on - strand at 9.326 kb on + strand at 9.327 kb on - strand at 9.431 kb on - strand at 9.483 kb on + strand at 9.581 kb on + strand at 9.607 kb on - strand at 9.646 kb on - strand at 9.646 kb on - strand at 9.829 kb on - strand, within GFF4892 at 9.996 kb on + strand, within GFF4892 at 10.072 kb on + strand, within GFF4892 at 10.163 kb on + strand, within GFF4892 at 10.417 kb on - strand, within GFF4892
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 6,887 + GFF4890 0.55 -1.6 6,887 + GFF4890 0.55 -2.1 6,888 - GFF4890 0.55 -1.5 6,888 - GFF4890 0.55 -2.9 7,204 + GFF4890 0.73 -0.9 7,358 + GFF4890 0.82 -1.0 7,359 - GFF4890 0.82 -1.1 7,377 + GFF4890 0.83 -1.8 7,492 + GFF4890 0.90 -1.7 7,493 - GFF4890 0.90 -0.1 7,495 - GFF4890 0.90 -2.1 7,495 - GFF4890 0.90 -1.9 7,617 - -1.0 7,627 + -1.0 7,651 - -1.9 7,672 - +0.6 7,672 - -0.1 8,012 + GFF4891 0.19 -2.0 8,108 + GFF4891 0.25 -1.7 8,136 - GFF4891 0.26 -2.3 8,233 - GFF4891 0.32 -0.1 8,249 + GFF4891 0.33 -2.9 8,252 + GFF4891 0.33 -2.1 8,369 - GFF4891 0.40 -0.1 8,372 - GFF4891 0.40 -1.7 8,372 - GFF4891 0.40 -1.8 8,372 - GFF4891 0.40 -1.6 8,372 - GFF4891 0.40 -1.9 8,470 + GFF4891 0.45 -1.8 8,497 + GFF4891 0.47 -2.4 8,506 - GFF4891 0.47 -2.1 8,657 - GFF4891 0.56 -1.1 8,899 + GFF4891 0.70 -2.0 9,027 + GFF4891 0.77 -0.7 9,027 + GFF4891 0.77 -1.7 9,030 + GFF4891 0.77 -1.5 9,030 + GFF4891 0.77 -2.5 9,040 + GFF4891 0.78 -1.5 9,041 - GFF4891 0.78 -1.7 9,283 + -1.6 9,283 + -2.0 9,283 + -2.4 9,284 - -2.4 9,326 + -1.6 9,327 - -1.3 9,431 - -2.4 9,483 + -1.4 9,581 + -0.0 9,607 - +0.2 9,646 - +0.2 9,646 - -1.1 9,829 - GFF4892 0.29 -0.4 9,996 + GFF4892 0.46 -0.3 10,072 + GFF4892 0.54 +1.2 10,163 + GFF4892 0.63 +1.5 10,417 - GFF4892 0.89 -0.3
Or see this region's nucleotide sequence