Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4855 and GFF4856 are separated by 16 nucleotides GFF4856 and GFF4857 are separated by 153 nucleotides GFF4857 and GFF4858 are separated by 233 nucleotides
GFF4855 - Lactaldehyde reductase (EC 1.1.1.77), at 154,470 to 155,618
GFF4855
GFF4856 - L-fuculose phosphate aldolase (EC 4.1.2.17), at 155,635 to 156,282
GFF4856
GFF4857 - FIG01045182: hypothetical protein, at 156,436 to 156,603
GFF4857
GFF4858 - Fucose permease, at 156,837 to 158,153
GFF4858
Position (kb) 155
156
157 Strain fitness (log2 ratio) -1
0
1 at 154.637 kb on + strand, within GFF4855 at 154.661 kb on + strand, within GFF4855 at 154.880 kb on - strand, within GFF4855 at 154.967 kb on + strand, within GFF4855 at 154.968 kb on - strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.009 kb on - strand, within GFF4855 at 155.018 kb on + strand, within GFF4855 at 155.167 kb on + strand, within GFF4855 at 155.217 kb on - strand, within GFF4855 at 155.288 kb on + strand, within GFF4855 at 155.351 kb on - strand, within GFF4855 at 155.647 kb on - strand at 155.738 kb on - strand, within GFF4856 at 155.738 kb on - strand, within GFF4856 at 155.742 kb on - strand, within GFF4856 at 155.946 kb on - strand, within GFF4856 at 155.946 kb on - strand, within GFF4856 at 156.007 kb on - strand, within GFF4856 at 156.781 kb on - strand at 156.907 kb on - strand at 156.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 154,637 + GFF4855 0.15 +0.3 154,661 + GFF4855 0.17 +0.0 154,880 - GFF4855 0.36 -0.1 154,967 + GFF4855 0.43 +0.1 154,968 - GFF4855 0.43 +0.3 154,971 + GFF4855 0.44 -0.2 154,971 + GFF4855 0.44 -0.1 154,972 - GFF4855 0.44 +0.8 154,972 - GFF4855 0.44 +0.6 154,972 - GFF4855 0.44 +0.3 154,972 - GFF4855 0.44 +0.6 154,972 - GFF4855 0.44 +0.0 155,007 - GFF4855 0.47 -0.3 155,007 - GFF4855 0.47 -0.1 155,009 - GFF4855 0.47 +0.1 155,018 + GFF4855 0.48 -0.3 155,167 + GFF4855 0.61 -0.4 155,217 - GFF4855 0.65 -0.1 155,288 + GFF4855 0.71 +1.4 155,351 - GFF4855 0.77 +0.3 155,647 - +1.1 155,738 - GFF4856 0.16 -0.1 155,738 - GFF4856 0.16 -0.2 155,742 - GFF4856 0.17 -0.7 155,946 - GFF4856 0.48 -1.0 155,946 - GFF4856 0.48 -0.4 156,007 - GFF4856 0.57 -0.3 156,781 - +1.0 156,907 - -0.2 156,907 - -0.3
Or see this region's nucleotide sequence