Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4732

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4731 and GFF4732 are separated by 202 nucleotidesGFF4732 and GFF4733 are separated by 384 nucleotides GFF4731 - Type III secretion injected virulence protein (YopP,YopJ, induces apoptosis, prevents cytokine induction, inhibits NFkb activation), at 39,681 to 40,547 GFF4731 GFF4732 - SPI1-associated transcriptional regulator SprB, at 40,750 to 41,505 GFF4732 GFF4733 - Type III secretion transcriptional regulator HilC (= SirC), at 41,890 to 42,777 GFF4733 Position (kb) 40 41 42Strain fitness (log2 ratio) -1 0 1at 39.811 kb on - strand, within GFF4731at 39.845 kb on - strand, within GFF4731at 39.873 kb on - strand, within GFF4731at 40.431 kb on - strand, within GFF4731at 40.806 kb on + strandat 41.098 kb on - strand, within GFF4732at 41.748 kb on - strandat 41.784 kb on - strandat 41.787 kb on + strandat 41.787 kb on + strandat 42.284 kb on + strand, within GFF4733

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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39,811 - GFF4731 0.15 -0.1
39,845 - GFF4731 0.19 +0.0
39,873 - GFF4731 0.22 -1.6
40,431 - GFF4731 0.87 +0.2
40,806 + +0.2
41,098 - GFF4732 0.46 -0.7
41,748 - -1.1
41,784 - +0.6
41,787 + -0.5
41,787 + -0.0
42,284 + GFF4733 0.44 +0.7

Or see this region's nucleotide sequence