Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4722

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4720 and GFF4721 are separated by 68 nucleotidesGFF4721 and GFF4722 overlap by 10 nucleotidesGFF4722 and GFF4723 overlap by 1 nucleotides GFF4720 - [NiFe] hydrogenase nickel incorporation protein HypA, at 29,578 to 29,934 GFF4720 GFF4721 - [NiFe] hydrogenase nickel incorporation-associated protein HypB, at 30,003 to 30,875 GFF4721 GFF4722 - [NiFe] hydrogenase metallocenter assembly protein HypC, at 30,866 to 31,138 GFF4722 GFF4723 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 31,138 to 32,259 GFF4723 Position (kb) 30 31 32Strain fitness (log2 ratio) -2 -1 0 1at 29.932 kb on + strandat 29.976 kb on + strandat 30.040 kb on - strandat 30.040 kb on - strandat 30.043 kb on + strandat 30.043 kb on + strandat 30.043 kb on + strandat 30.043 kb on + strandat 30.044 kb on - strandat 30.120 kb on - strand, within GFF4721at 30.131 kb on + strand, within GFF4721at 30.266 kb on + strand, within GFF4721at 30.398 kb on - strand, within GFF4721at 30.627 kb on - strand, within GFF4721at 31.084 kb on + strand, within GFF4722at 31.136 kb on + strandat 31.137 kb on - strandat 31.157 kb on - strandat 31.305 kb on - strand, within GFF4723at 31.497 kb on + strand, within GFF4723at 31.691 kb on + strand, within GFF4723at 31.833 kb on - strand, within GFF4723at 31.833 kb on - strand, within GFF4723at 31.897 kb on + strand, within GFF4723at 32.083 kb on + strand, within GFF4723at 32.084 kb on - strand, within GFF4723at 32.084 kb on - strand, within GFF4723

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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29,932 + +0.0
29,976 + -1.8
30,040 - -0.7
30,040 - +1.0
30,043 + +0.3
30,043 + +0.1
30,043 + +0.4
30,043 + -0.2
30,044 - -0.1
30,120 - GFF4721 0.13 +0.1
30,131 + GFF4721 0.15 +0.0
30,266 + GFF4721 0.30 -0.2
30,398 - GFF4721 0.45 +0.2
30,627 - GFF4721 0.71 +0.2
31,084 + GFF4722 0.80 -0.9
31,136 + -0.7
31,137 - +0.9
31,157 - -0.1
31,305 - GFF4723 0.15 +0.2
31,497 + GFF4723 0.32 -0.3
31,691 + GFF4723 0.49 -1.2
31,833 - GFF4723 0.62 -0.3
31,833 - GFF4723 0.62 +0.5
31,897 + GFF4723 0.68 +0.5
32,083 + GFF4723 0.84 -1.2
32,084 - GFF4723 0.84 -0.0
32,084 - GFF4723 0.84 +0.2

Or see this region's nucleotide sequence