Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4720 and GFF4721 are separated by 68 nucleotides GFF4721 and GFF4722 overlap by 10 nucleotides GFF4722 and GFF4723 overlap by 1 nucleotides
GFF4720 - [NiFe] hydrogenase nickel incorporation protein HypA, at 29,578 to 29,934
GFF4720
GFF4721 - [NiFe] hydrogenase nickel incorporation-associated protein HypB, at 30,003 to 30,875
GFF4721
GFF4722 - [NiFe] hydrogenase metallocenter assembly protein HypC, at 30,866 to 31,138
GFF4722
GFF4723 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 31,138 to 32,259
GFF4723
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.932 kb on + strand at 29.976 kb on + strand at 30.040 kb on - strand at 30.040 kb on - strand at 30.043 kb on + strand at 30.043 kb on + strand at 30.043 kb on + strand at 30.043 kb on + strand at 30.044 kb on - strand at 30.120 kb on - strand, within GFF4721 at 30.131 kb on + strand, within GFF4721 at 30.266 kb on + strand, within GFF4721 at 30.398 kb on - strand, within GFF4721 at 30.627 kb on - strand, within GFF4721 at 31.084 kb on + strand, within GFF4722 at 31.136 kb on + strand at 31.137 kb on - strand at 31.157 kb on - strand at 31.305 kb on - strand, within GFF4723 at 31.497 kb on + strand, within GFF4723 at 31.691 kb on + strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.897 kb on + strand, within GFF4723 at 32.083 kb on + strand, within GFF4723 at 32.084 kb on - strand, within GFF4723 at 32.084 kb on - strand, within GFF4723
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 29,932 + +0.0 29,976 + -1.8 30,040 - -0.7 30,040 - +1.0 30,043 + +0.3 30,043 + +0.1 30,043 + +0.4 30,043 + -0.2 30,044 - -0.1 30,120 - GFF4721 0.13 +0.1 30,131 + GFF4721 0.15 +0.0 30,266 + GFF4721 0.30 -0.2 30,398 - GFF4721 0.45 +0.2 30,627 - GFF4721 0.71 +0.2 31,084 + GFF4722 0.80 -0.9 31,136 + -0.7 31,137 - +0.9 31,157 - -0.1 31,305 - GFF4723 0.15 +0.2 31,497 + GFF4723 0.32 -0.3 31,691 + GFF4723 0.49 -1.2 31,833 - GFF4723 0.62 -0.3 31,833 - GFF4723 0.62 +0.5 31,897 + GFF4723 0.68 +0.5 32,083 + GFF4723 0.84 -1.2 32,084 - GFF4723 0.84 -0.0 32,084 - GFF4723 0.84 +0.2
Or see this region's nucleotide sequence