Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4612 and GFF4613 are separated by 38 nucleotides GFF4613 and GFF4614 are separated by 43 nucleotides GFF4614 and GFF4615 are separated by 171 nucleotides
GFF4612 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 405,322 to 406,743
GFF4612
GFF4613 - Di/tripeptide permease YjdL, at 406,782 to 408,263
GFF4613
GFF4614 - hypothetical protein, at 408,307 to 408,420
GFF4614
GFF4615 - FIG137478: Hypothetical protein YbgI, at 408,592 to 409,335
GFF4615
Position (kb) 406
407
408
409 Strain fitness (log2 ratio) -2
-1
0
1 at 405.783 kb on + strand, within GFF4612 at 405.867 kb on + strand, within GFF4612 at 405.891 kb on + strand, within GFF4612 at 405.892 kb on - strand, within GFF4612 at 406.365 kb on + strand, within GFF4612 at 406.366 kb on - strand, within GFF4612 at 406.524 kb on + strand, within GFF4612 at 406.524 kb on + strand, within GFF4612 at 406.745 kb on - strand at 406.745 kb on - strand at 406.780 kb on + strand at 406.780 kb on + strand at 406.981 kb on + strand, within GFF4613 at 406.982 kb on - strand, within GFF4613 at 406.982 kb on - strand, within GFF4613 at 406.986 kb on - strand, within GFF4613 at 407.010 kb on - strand, within GFF4613 at 407.058 kb on - strand, within GFF4613 at 407.235 kb on - strand, within GFF4613 at 407.257 kb on - strand, within GFF4613 at 407.337 kb on - strand, within GFF4613 at 407.382 kb on - strand, within GFF4613 at 407.393 kb on + strand, within GFF4613 at 407.393 kb on + strand, within GFF4613 at 407.394 kb on - strand, within GFF4613 at 407.552 kb on - strand, within GFF4613 at 407.642 kb on - strand, within GFF4613 at 407.732 kb on + strand, within GFF4613 at 407.834 kb on - strand, within GFF4613 at 407.900 kb on + strand, within GFF4613 at 407.900 kb on + strand, within GFF4613 at 408.000 kb on + strand, within GFF4613 at 408.000 kb on + strand, within GFF4613 at 408.001 kb on - strand, within GFF4613 at 408.053 kb on + strand, within GFF4613 at 408.089 kb on - strand, within GFF4613 at 408.144 kb on + strand at 408.144 kb on + strand at 408.183 kb on - strand at 408.187 kb on + strand at 408.187 kb on + strand at 408.226 kb on + strand at 408.229 kb on + strand at 408.333 kb on + strand, within GFF4614 at 408.532 kb on + strand at 408.566 kb on + strand at 408.578 kb on + strand at 409.126 kb on - strand, within GFF4615 at 409.126 kb on - strand, within GFF4615
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 405,783 + GFF4612 0.32 +0.2 405,867 + GFF4612 0.38 +0.6 405,891 + GFF4612 0.40 -1.0 405,892 - GFF4612 0.40 +0.7 406,365 + GFF4612 0.73 +0.2 406,366 - GFF4612 0.73 -0.2 406,524 + GFF4612 0.85 +0.9 406,524 + GFF4612 0.85 -0.3 406,745 - -0.4 406,745 - -0.5 406,780 + -0.2 406,780 + +0.5 406,981 + GFF4613 0.13 +0.4 406,982 - GFF4613 0.13 +0.8 406,982 - GFF4613 0.13 -0.0 406,986 - GFF4613 0.14 +0.0 407,010 - GFF4613 0.15 +0.5 407,058 - GFF4613 0.19 +0.9 407,235 - GFF4613 0.31 -0.1 407,257 - GFF4613 0.32 +0.6 407,337 - GFF4613 0.37 +0.9 407,382 - GFF4613 0.40 +0.0 407,393 + GFF4613 0.41 -0.2 407,393 + GFF4613 0.41 -0.1 407,394 - GFF4613 0.41 -1.9 407,552 - GFF4613 0.52 -0.8 407,642 - GFF4613 0.58 +1.4 407,732 + GFF4613 0.64 -0.1 407,834 - GFF4613 0.71 +0.4 407,900 + GFF4613 0.75 +0.0 407,900 + GFF4613 0.75 -0.8 408,000 + GFF4613 0.82 +0.9 408,000 + GFF4613 0.82 +0.4 408,001 - GFF4613 0.82 +0.2 408,053 + GFF4613 0.86 +0.1 408,089 - GFF4613 0.88 -0.5 408,144 + -1.4 408,144 + +0.2 408,183 - +0.2 408,187 + +0.1 408,187 + -0.1 408,226 + -0.2 408,229 + +0.2 408,333 + GFF4614 0.23 +0.4 408,532 + +0.7 408,566 + +0.4 408,578 + +0.1 409,126 - GFF4615 0.72 -0.6 409,126 - GFF4615 0.72 -1.7
Or see this region's nucleotide sequence