Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4543 and GFF4544 are separated by 99 nucleotides GFF4544 and GFF4545 are separated by 108 nucleotides GFF4545 and GFF4546 are separated by 139 nucleotides
GFF4543 - Octanoate-[acyl-carrier-protein]-protein-N-octan oyltransferase, at 327,502 to 328,143
GFF4543
GFF4544 - Proposed lipoate regulatory protein YbeD, at 328,243 to 328,506
GFF4544
GFF4545 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 328,615 to 329,826
GFF4545
GFF4546 - Rare lipoprotein A precursor, at 329,966 to 331,111
GFF4546
Position (kb) 328
329
330 Strain fitness (log2 ratio) -1
0
1
2
3 at 328.154 kb on - strand at 328.183 kb on - strand at 328.183 kb on - strand at 328.185 kb on - strand at 328.244 kb on + strand at 328.245 kb on - strand at 328.245 kb on - strand at 328.522 kb on - strand at 328.574 kb on - strand at 328.577 kb on - strand at 328.577 kb on - strand at 328.578 kb on + strand at 328.579 kb on - strand at 328.802 kb on - strand, within GFF4545 at 328.805 kb on + strand, within GFF4545 at 328.805 kb on + strand, within GFF4545 at 329.005 kb on - strand, within GFF4545 at 329.007 kb on - strand, within GFF4545 at 329.291 kb on - strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.491 kb on - strand, within GFF4545 at 329.585 kb on + strand, within GFF4545 at 329.800 kb on - strand at 329.837 kb on - strand at 329.870 kb on + strand at 329.875 kb on + strand at 329.886 kb on + strand at 329.994 kb on - strand at 330.043 kb on + strand at 330.090 kb on - strand, within GFF4546 at 330.122 kb on + strand, within GFF4546 at 330.439 kb on + strand, within GFF4546 at 330.507 kb on - strand, within GFF4546 at 330.615 kb on - strand, within GFF4546 at 330.619 kb on + strand, within GFF4546 at 330.625 kb on + strand, within GFF4546 at 330.753 kb on + strand, within GFF4546
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 328,154 - +1.0 328,183 - +0.1 328,183 - -1.2 328,185 - -0.2 328,244 + -0.1 328,245 - -0.3 328,245 - +0.4 328,522 - +0.3 328,574 - +1.1 328,577 - +0.4 328,577 - +0.2 328,578 + -0.9 328,579 - +0.6 328,802 - GFF4545 0.15 -0.4 328,805 + GFF4545 0.16 +0.3 328,805 + GFF4545 0.16 +0.2 329,005 - GFF4545 0.32 -0.5 329,007 - GFF4545 0.32 +0.4 329,291 - GFF4545 0.56 +0.1 329,333 + GFF4545 0.59 -0.1 329,333 + GFF4545 0.59 -0.3 329,333 + GFF4545 0.59 +3.2 329,334 - GFF4545 0.59 +0.2 329,334 - GFF4545 0.59 +1.0 329,334 - GFF4545 0.59 -0.1 329,334 - GFF4545 0.59 -0.4 329,334 - GFF4545 0.59 +0.1 329,491 - GFF4545 0.72 -0.6 329,585 + GFF4545 0.80 -0.3 329,800 - -0.1 329,837 - +0.1 329,870 + -0.0 329,875 + -0.2 329,886 + -0.7 329,994 - -0.3 330,043 + -0.6 330,090 - GFF4546 0.11 -0.2 330,122 + GFF4546 0.14 -0.0 330,439 + GFF4546 0.41 +0.3 330,507 - GFF4546 0.47 -1.4 330,615 - GFF4546 0.57 -0.1 330,619 + GFF4546 0.57 +0.3 330,625 + GFF4546 0.58 -0.5 330,753 + GFF4546 0.69 -0.1
Or see this region's nucleotide sequence