Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF453

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF452 and GFF453 are separated by 844 nucleotidesGFF453 and GFF454 are separated by 173 nucleotidesGFF454 and GFF455 are separated by 288 nucleotides GFF452 - FIG032766: hypothetical protein, at 174,603 to 174,869 GFF452 GFF453 - FIG00923183: possible membrane protein YebY, at 175,714 to 176,253 GFF453 GFF454 - Cold shock protein CspA, at 176,427 to 176,639 GFF454 GFF455 - FIG00638451: hypothetical protein, at 176,928 to 177,218 GFF455 Position (kb) 175 176 177Strain fitness (log2 ratio) -2 -1 0 1at 175.118 kb on - strandat 175.119 kb on + strandat 175.120 kb on - strandat 175.227 kb on + strandat 175.253 kb on - strandat 175.328 kb on - strandat 175.328 kb on - strandat 175.341 kb on - strandat 175.398 kb on - strandat 175.418 kb on + strandat 175.540 kb on + strandat 175.799 kb on - strand, within GFF453at 176.031 kb on + strand, within GFF453at 176.053 kb on + strand, within GFF453at 176.233 kb on + strandat 176.253 kb on + strandat 176.286 kb on + strandat 176.336 kb on - strandat 176.559 kb on - strand, within GFF454at 176.577 kb on - strand, within GFF454at 176.580 kb on - strand, within GFF454at 176.614 kb on - strand, within GFF454at 176.640 kb on - strandat 176.640 kb on - strandat 176.645 kb on - strandat 176.648 kb on - strandat 176.768 kb on - strandat 176.848 kb on + strandat 176.894 kb on - strandat 176.949 kb on + strandat 176.950 kb on - strandat 177.097 kb on - strand, within GFF455at 177.209 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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175,118 - -0.4
175,119 + +0.4
175,120 - +0.0
175,227 + +0.1
175,253 - -0.3
175,328 - +0.5
175,328 - +0.3
175,341 - +0.1
175,398 - -0.0
175,418 + +0.8
175,540 + -0.2
175,799 - GFF453 0.16 -0.1
176,031 + GFF453 0.59 -0.4
176,053 + GFF453 0.63 -1.9
176,233 + -0.8
176,253 + +0.1
176,286 + -0.1
176,336 - +1.1
176,559 - GFF454 0.62 +0.7
176,577 - GFF454 0.70 -1.1
176,580 - GFF454 0.72 +0.6
176,614 - GFF454 0.88 -0.1
176,640 - -0.3
176,640 - -0.2
176,645 - -0.8
176,648 - -0.2
176,768 - +0.5
176,848 + +0.5
176,894 - +0.1
176,949 + -0.6
176,950 - +0.4
177,097 - GFF455 0.58 +0.0
177,209 + +1.2

Or see this region's nucleotide sequence