Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4336 and GFF4337 are separated by 218 nucleotides GFF4337 and GFF4338 are separated by 46 nucleotides
GFF4335 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 116,439 to 117,029
GFF4335
GFF4336 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 116,992 to 117,810
GFF4336
GFF4337 - FIG001943: hypothetical protein YajQ, at 118,029 to 118,520
GFF4337
GFF4338 - Putative transport protein, at 118,567 to 119,931
GFF4338
Position (kb)
118
119 Strain fitness (log2 ratio)
-1
0
1 at 117.117 kb on - strand, within GFF4336 at 117.216 kb on - strand, within GFF4336 at 117.303 kb on + strand, within GFF4336 at 117.304 kb on - strand, within GFF4336 at 117.427 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.490 kb on - strand, within GFF4336 at 117.495 kb on + strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.526 kb on - strand, within GFF4336 at 117.553 kb on - strand, within GFF4336 at 117.580 kb on - strand, within GFF4336 at 117.612 kb on + strand, within GFF4336 at 117.704 kb on - strand, within GFF4336 at 117.798 kb on - strand at 117.826 kb on + strand at 117.826 kb on + strand at 117.950 kb on + strand at 117.991 kb on + strand at 117.992 kb on - strand at 118.163 kb on + strand, within GFF4337 at 118.773 kb on + strand, within GFF4338 at 118.792 kb on + strand, within GFF4338 at 118.904 kb on + strand, within GFF4338 at 118.954 kb on - strand, within GFF4338 at 119.124 kb on - strand, within GFF4338 at 119.124 kb on - strand, within GFF4338 at 119.252 kb on - strand, within GFF4338 at 119.268 kb on + strand, within GFF4338 at 119.269 kb on - strand, within GFF4338 at 119.269 kb on - strand, within GFF4338 at 119.314 kb on - strand, within GFF4338 at 119.452 kb on - strand, within GFF4338 at 119.473 kb on + strand, within GFF4338 at 119.496 kb on + strand, within GFF4338
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 117,117 - GFF4336 0.15 -0.5 117,216 - GFF4336 0.27 -0.4 117,303 + GFF4336 0.38 +0.1 117,304 - GFF4336 0.38 -0.3 117,427 + GFF4336 0.53 +1.1 117,489 + GFF4336 0.61 +1.3 117,489 + GFF4336 0.61 -0.0 117,490 - GFF4336 0.61 +0.5 117,495 + GFF4336 0.61 +0.9 117,496 - GFF4336 0.62 +0.1 117,496 - GFF4336 0.62 +1.4 117,526 - GFF4336 0.65 +0.9 117,553 - GFF4336 0.68 +0.4 117,580 - GFF4336 0.72 +0.5 117,612 + GFF4336 0.76 -0.5 117,704 - GFF4336 0.87 -1.5 117,798 - +0.1 117,826 + +0.8 117,826 + -0.6 117,950 + -0.1 117,991 + +0.8 117,992 - -0.2 118,163 + GFF4337 0.27 -0.5 118,773 + GFF4338 0.15 -0.2 118,792 + GFF4338 0.16 -1.1 118,904 + GFF4338 0.25 +0.7 118,954 - GFF4338 0.28 +0.1 119,124 - GFF4338 0.41 -0.0 119,124 - GFF4338 0.41 -0.2 119,252 - GFF4338 0.50 -0.5 119,268 + GFF4338 0.51 -0.3 119,269 - GFF4338 0.51 -0.1 119,269 - GFF4338 0.51 -0.2 119,314 - GFF4338 0.55 +0.2 119,452 - GFF4338 0.65 -1.2 119,473 + GFF4338 0.66 -0.4 119,496 + GFF4338 0.68 +0.4
Or see this region's nucleotide sequence