Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4206

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4203 and GFF4204 are separated by 150 nucleotidesGFF4204 and GFF4205 are separated by 3 nucleotidesGFF4205 and GFF4206 are separated by 232 nucleotidesGFF4206 and GFF4207 are separated by 289 nucleotidesGFF4207 and GFF4208 overlap by 23 nucleotidesGFF4208 and GFF4209 are separated by 5 nucleotides GFF4203 - Thiazole biosynthesis protein ThiH, at 16,430 to 17,563 GFF4203 GFF4204 - putative cytoplasmic protein, at 17,714 to 17,860 GFF4204 GFF4205 - hypothetical protein, at 17,864 to 17,992 GFF4205 GFF4206 - putative cytoplasmic protein, at 18,225 to 19,253 GFF4206 GFF4207 - FIG01046757: hypothetical protein, at 19,543 to 19,680 GFF4207 GFF4208 - FIG01046757: hypothetical protein, at 19,658 to 19,978 GFF4208 GFF4209 - STMF1.14 protein, at 19,984 to 20,262 GFF4209 Position (kb) 18 19 20Strain fitness (log2 ratio) -1 0 1at 17.225 kb on - strand, within GFF4203at 17.317 kb on - strand, within GFF4203at 17.569 kb on + strandat 17.570 kb on - strandat 17.582 kb on + strandat 17.586 kb on - strandat 17.604 kb on + strandat 17.604 kb on + strandat 17.604 kb on + strandat 17.688 kb on - strandat 17.701 kb on + strandat 17.944 kb on - strand, within GFF4205at 17.944 kb on - strand, within GFF4205at 18.270 kb on - strandat 18.492 kb on + strand, within GFF4206at 18.654 kb on - strand, within GFF4206at 19.214 kb on - strandat 19.864 kb on + strand, within GFF4208

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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17,225 - GFF4203 0.70 -0.2
17,317 - GFF4203 0.78 -0.1
17,569 + -0.2
17,570 - +0.4
17,582 + -0.0
17,586 - +0.3
17,604 + -0.1
17,604 + -0.0
17,604 + -0.2
17,688 - +0.5
17,701 + -0.5
17,944 - GFF4205 0.62 -0.7
17,944 - GFF4205 0.62 -0.7
18,270 - -0.4
18,492 + GFF4206 0.26 -1.1
18,654 - GFF4206 0.42 -0.1
19,214 - +1.0
19,864 + GFF4208 0.64 +0.3

Or see this region's nucleotide sequence