Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4203 and GFF4204 are separated by 150 nucleotides GFF4204 and GFF4205 are separated by 3 nucleotides GFF4205 and GFF4206 are separated by 232 nucleotides GFF4206 and GFF4207 are separated by 289 nucleotides GFF4207 and GFF4208 overlap by 23 nucleotides GFF4208 and GFF4209 are separated by 5 nucleotides
GFF4203 - Thiazole biosynthesis protein ThiH, at 16,430 to 17,563
GFF4203
GFF4204 - putative cytoplasmic protein, at 17,714 to 17,860
GFF4204
GFF4205 - hypothetical protein, at 17,864 to 17,992
GFF4205
GFF4206 - putative cytoplasmic protein, at 18,225 to 19,253
GFF4206
GFF4207 - FIG01046757: hypothetical protein, at 19,543 to 19,680
GFF4207
GFF4208 - FIG01046757: hypothetical protein, at 19,658 to 19,978
GFF4208
GFF4209 - STMF1.14 protein, at 19,984 to 20,262
GFF4209
Position (kb)
18
19
20 Strain fitness (log2 ratio)
-1
0
1 at 17.225 kb on - strand, within GFF4203 at 17.317 kb on - strand, within GFF4203 at 17.569 kb on + strand at 17.570 kb on - strand at 17.582 kb on + strand at 17.586 kb on - strand at 17.604 kb on + strand at 17.604 kb on + strand at 17.604 kb on + strand at 17.688 kb on - strand at 17.701 kb on + strand at 17.944 kb on - strand, within GFF4205 at 17.944 kb on - strand, within GFF4205 at 18.270 kb on - strand at 18.492 kb on + strand, within GFF4206 at 18.654 kb on - strand, within GFF4206 at 19.214 kb on - strand at 19.864 kb on + strand, within GFF4208
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 17,225 - GFF4203 0.70 -0.2 17,317 - GFF4203 0.78 -0.1 17,569 + -0.2 17,570 - +0.4 17,582 + -0.0 17,586 - +0.3 17,604 + -0.1 17,604 + -0.0 17,604 + -0.2 17,688 - +0.5 17,701 + -0.5 17,944 - GFF4205 0.62 -0.7 17,944 - GFF4205 0.62 -0.7 18,270 - -0.4 18,492 + GFF4206 0.26 -1.1 18,654 - GFF4206 0.42 -0.1 19,214 - +1.0 19,864 + GFF4208 0.64 +0.3
Or see this region's nucleotide sequence