Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4144 and GFF4145 are separated by 257 nucleotides GFF4145 and GFF4146 are separated by 119 nucleotides GFF4146 and GFF4147 are separated by 35 nucleotides GFF4147 and GFF4148 are separated by 465 nucleotides
GFF4144 - putative ATPase, at 23,833 to 25,116
GFF4144
GFF4145 - hypothetical protein, at 25,374 to 25,499
GFF4145
GFF4146 - putative inner membrane protein, at 25,619 to 27,688
GFF4146
GFF4147 - putative cytoplasmic protein, at 27,724 to 27,939
GFF4147
GFF4148 - putative cytoplasmic protein, at 28,405 to 29,217
GFF4148
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.276 kb on - strand at 25.394 kb on + strand, within GFF4145 at 25.413 kb on - strand, within GFF4145 at 25.505 kb on - strand at 25.530 kb on + strand at 25.546 kb on - strand at 25.609 kb on + strand at 25.740 kb on - strand at 26.165 kb on + strand, within GFF4146 at 26.166 kb on - strand, within GFF4146 at 26.304 kb on - strand, within GFF4146 at 26.349 kb on - strand, within GFF4146 at 26.377 kb on + strand, within GFF4146 at 26.377 kb on + strand, within GFF4146 at 26.377 kb on + strand, within GFF4146 at 26.513 kb on - strand, within GFF4146 at 26.513 kb on - strand, within GFF4146 at 26.514 kb on + strand, within GFF4146 at 26.868 kb on + strand, within GFF4146 at 26.931 kb on + strand, within GFF4146 at 26.932 kb on - strand, within GFF4146 at 26.932 kb on - strand, within GFF4146 at 26.934 kb on - strand, within GFF4146 at 27.001 kb on - strand, within GFF4146 at 27.001 kb on - strand, within GFF4146 at 27.166 kb on - strand, within GFF4146 at 27.978 kb on - strand at 28.189 kb on + strand at 28.190 kb on - strand at 28.216 kb on + strand at 28.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 25,276 - +0.2 25,394 + GFF4145 0.16 -0.4 25,413 - GFF4145 0.31 -2.0 25,505 - -1.7 25,530 + -0.8 25,546 - -0.1 25,609 + +0.2 25,740 - -1.4 26,165 + GFF4146 0.26 -0.6 26,166 - GFF4146 0.26 +0.6 26,304 - GFF4146 0.33 +0.7 26,349 - GFF4146 0.35 -3.1 26,377 + GFF4146 0.37 -1.0 26,377 + GFF4146 0.37 +0.3 26,377 + GFF4146 0.37 +1.6 26,513 - GFF4146 0.43 +0.5 26,513 - GFF4146 0.43 +0.3 26,514 + GFF4146 0.43 +1.3 26,868 + GFF4146 0.60 -0.4 26,931 + GFF4146 0.63 +0.4 26,932 - GFF4146 0.63 +0.4 26,932 - GFF4146 0.63 -0.1 26,934 - GFF4146 0.64 -0.5 27,001 - GFF4146 0.67 +0.8 27,001 - GFF4146 0.67 +0.4 27,166 - GFF4146 0.75 -0.5 27,978 - +0.6 28,189 + -0.0 28,190 - +0.5 28,216 + -0.0 28,280 - -0.1
Or see this region's nucleotide sequence