Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4002 and GFF4003 are separated by 309 nucleotides GFF4003 and GFF4004 are separated by 116 nucleotides GFF4004 and GFF4005 are separated by 38 nucleotides GFF4005 and GFF4006 are separated by 281 nucleotides
GFF4002 - Putative mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70), at 275,089 to 275,904
GFF4002
GFF4003 - putative cytoplasmic protein, at 276,214 to 276,450
GFF4003
GFF4004 - DsrB protein, at 276,567 to 276,764
GFF4004
GFF4005 - Colanic acid capsular biosynthesis activation accesory protein RcsA, co-regulator with RcsB, at 276,803 to 277,426
GFF4005
GFF4006 - Flagellar biosynthesis protein FliR, at 277,708 to 278,502
GFF4006
Position (kb) 276
277 Strain fitness (log2 ratio) -2
-1
0
1
2 at 275.590 kb on - strand, within GFF4002 at 275.642 kb on + strand, within GFF4002 at 275.643 kb on - strand, within GFF4002 at 275.643 kb on - strand, within GFF4002 at 275.717 kb on - strand, within GFF4002 at 275.722 kb on + strand, within GFF4002 at 276.112 kb on + strand at 276.112 kb on + strand at 276.164 kb on + strand at 276.223 kb on + strand at 276.249 kb on + strand, within GFF4003 at 276.285 kb on + strand, within GFF4003 at 276.285 kb on + strand, within GFF4003 at 276.285 kb on + strand, within GFF4003 at 276.286 kb on - strand, within GFF4003 at 276.290 kb on + strand, within GFF4003 at 276.385 kb on + strand, within GFF4003 at 276.475 kb on + strand at 276.539 kb on + strand at 276.666 kb on + strand, within GFF4004 at 276.762 kb on + strand at 276.855 kb on - strand at 276.862 kb on - strand at 277.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 275,590 - GFF4002 0.61 +0.1 275,642 + GFF4002 0.68 -0.4 275,643 - GFF4002 0.68 +0.1 275,643 - GFF4002 0.68 +0.5 275,717 - GFF4002 0.77 -0.7 275,722 + GFF4002 0.78 +0.0 276,112 + -0.8 276,112 + +0.0 276,164 + +0.4 276,223 + +0.1 276,249 + GFF4003 0.15 -0.2 276,285 + GFF4003 0.30 +0.5 276,285 + GFF4003 0.30 -2.0 276,285 + GFF4003 0.30 +2.4 276,286 - GFF4003 0.30 +0.4 276,290 + GFF4003 0.32 -0.6 276,385 + GFF4003 0.72 -0.4 276,475 + +0.4 276,539 + +0.8 276,666 + GFF4004 0.50 -0.4 276,762 + +0.5 276,855 - -0.3 276,862 - -0.1 277,395 + -0.2
Or see this region's nucleotide sequence