Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3942 and GFF3943 are separated by 9 nucleotides GFF3943 and GFF3944 are separated by 14 nucleotides GFF3944 and GFF3945 are separated by 10 nucleotides
GFF3942 - Propanediol dehydratase reactivation factor large subunit, at 222,168 to 224,000
GFF3942
GFF3943 - Propanediol dehydratase small subunit (EC 4.2.1.28), at 224,010 to 224,531
GFF3943
GFF3944 - Propanediol dehydratase medium subunit (EC 4.2.1.28), at 224,546 to 225,220
GFF3944
GFF3945 - Propanediol dehydratase large subunit (EC 4.2.1.28), at 225,231 to 226,895
GFF3945
Position (kb) 224
225 Strain fitness (log2 ratio) -1
0
1
2 at 223.260 kb on - strand, within GFF3942 at 223.441 kb on + strand, within GFF3942 at 223.526 kb on + strand, within GFF3942 at 223.913 kb on + strand at 223.971 kb on - strand at 223.991 kb on + strand at 223.992 kb on - strand at 223.992 kb on - strand at 223.994 kb on - strand at 224.045 kb on - strand at 224.164 kb on + strand, within GFF3943 at 224.493 kb on + strand at 224.493 kb on + strand at 224.538 kb on - strand at 224.592 kb on + strand at 224.606 kb on - strand at 224.609 kb on - strand at 224.635 kb on - strand, within GFF3944 at 224.728 kb on - strand, within GFF3944 at 224.812 kb on + strand, within GFF3944 at 224.812 kb on + strand, within GFF3944 at 224.813 kb on - strand, within GFF3944 at 225.189 kb on - strand at 225.500 kb on + strand, within GFF3945
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 223,260 - GFF3942 0.60 +0.6 223,441 + GFF3942 0.69 -0.5 223,526 + GFF3942 0.74 -0.7 223,913 + -0.2 223,971 - -1.2 223,991 + -0.0 223,992 - +0.3 223,992 - +0.1 223,994 - -0.0 224,045 - +0.9 224,164 + GFF3943 0.30 -1.4 224,493 + +2.2 224,493 + +1.2 224,538 - +0.4 224,592 + +0.2 224,606 - -0.9 224,609 - +0.9 224,635 - GFF3944 0.13 -0.7 224,728 - GFF3944 0.27 +0.3 224,812 + GFF3944 0.39 -0.2 224,812 + GFF3944 0.39 -1.5 224,813 - GFF3944 0.40 +0.0 225,189 - +0.5 225,500 + GFF3945 0.16 +1.2
Or see this region's nucleotide sequence