Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3928 and GFF3929 overlap by 16 nucleotides GFF3929 and GFF3930 are separated by 16 nucleotides GFF3930 and GFF3931 overlap by 1 nucleotides GFF3931 and GFF3932 are separated by 2 nucleotides
GFF3928 - Propionate kinase, propanediol utilization (EC 2.7.2.1), at 212,199 to 213,413
GFF3928
GFF3929 - Propanediol utilization protein PduV, at 213,398 to 213,838
GFF3929
GFF3930 - Propanediol utilization polyhedral body protein PduU, at 213,855 to 214,205
GFF3930
GFF3931 - Propanediol utilization polyhedral body protein PduT, at 214,205 to 214,759
GFF3931
GFF3932 - Cob(III)alamin reductase @ Cob(II)alamin reductase, at 214,762 to 216,117
GFF3932
Position (kb) 214
215 Strain fitness (log2 ratio) -1
0
1 at 213.278 kb on + strand, within GFF3928 at 213.279 kb on - strand, within GFF3928 at 213.508 kb on + strand, within GFF3929 at 213.532 kb on + strand, within GFF3929 at 213.691 kb on - strand, within GFF3929 at 213.712 kb on - strand, within GFF3929 at 213.769 kb on - strand, within GFF3929 at 213.857 kb on - strand at 214.165 kb on + strand, within GFF3930 at 214.166 kb on - strand, within GFF3930 at 214.351 kb on - strand, within GFF3931 at 214.357 kb on + strand, within GFF3931 at 214.358 kb on - strand, within GFF3931 at 214.358 kb on - strand, within GFF3931 at 214.379 kb on - strand, within GFF3931 at 214.517 kb on + strand, within GFF3931 at 214.667 kb on + strand, within GFF3931 at 214.772 kb on - strand at 214.926 kb on + strand, within GFF3932 at 215.108 kb on - strand, within GFF3932 at 215.485 kb on - strand, within GFF3932 at 215.527 kb on + strand, within GFF3932 at 215.527 kb on + strand, within GFF3932 at 215.665 kb on - strand, within GFF3932
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 213,278 + GFF3928 0.89 -0.5 213,279 - GFF3928 0.89 -0.4 213,508 + GFF3929 0.25 +0.1 213,532 + GFF3929 0.30 -0.6 213,691 - GFF3929 0.66 +1.1 213,712 - GFF3929 0.71 -0.2 213,769 - GFF3929 0.84 -0.1 213,857 - +0.8 214,165 + GFF3930 0.88 +0.4 214,166 - GFF3930 0.89 +0.1 214,351 - GFF3931 0.26 -0.3 214,357 + GFF3931 0.27 -0.3 214,358 - GFF3931 0.28 -0.2 214,358 - GFF3931 0.28 -0.6 214,379 - GFF3931 0.31 -0.3 214,517 + GFF3931 0.56 -0.1 214,667 + GFF3931 0.83 -0.7 214,772 - +0.1 214,926 + GFF3932 0.12 -0.4 215,108 - GFF3932 0.26 -0.5 215,485 - GFF3932 0.53 +0.3 215,527 + GFF3932 0.56 +0.0 215,527 + GFF3932 0.56 +1.2 215,665 - GFF3932 0.67 -0.2
Or see this region's nucleotide sequence