Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3819 and GFF3820 overlap by 4 nucleotides GFF3820 and GFF3821 are separated by 46 nucleotides GFF3821 and GFF3822 are separated by 56 nucleotides
GFF3819 - Autolysis histidine kinase LytS, at 88,707 to 90,392
GFF3819
GFF3820 - Two-component response-regulatory protein YehT, at 90,389 to 91,108
GFF3820
GFF3821 - FIG00638377: hypothetical protein, at 91,155 to 91,622
GFF3821
GFF3822 - FIG00545237: hypothetical protein, at 91,679 to 92,101
GFF3822
Position (kb) 90
91
92 Strain fitness (log2 ratio) -1
0
1
2 at 89.402 kb on + strand, within GFF3819 at 89.422 kb on + strand, within GFF3819 at 89.423 kb on - strand, within GFF3819 at 89.537 kb on + strand, within GFF3819 at 89.537 kb on + strand, within GFF3819 at 89.537 kb on + strand, within GFF3819 at 89.685 kb on - strand, within GFF3819 at 89.685 kb on - strand, within GFF3819 at 89.724 kb on - strand, within GFF3819 at 89.784 kb on + strand, within GFF3819 at 89.874 kb on - strand, within GFF3819 at 90.004 kb on + strand, within GFF3819 at 90.134 kb on + strand, within GFF3819 at 90.216 kb on - strand, within GFF3819 at 90.257 kb on + strand at 90.324 kb on - strand at 90.360 kb on - strand at 90.365 kb on - strand at 90.520 kb on + strand, within GFF3820 at 90.520 kb on + strand, within GFF3820 at 90.724 kb on - strand, within GFF3820 at 90.866 kb on - strand, within GFF3820 at 90.982 kb on + strand, within GFF3820 at 91.205 kb on + strand, within GFF3821 at 91.205 kb on + strand, within GFF3821 at 91.415 kb on + strand, within GFF3821 at 91.415 kb on + strand, within GFF3821 at 91.416 kb on - strand, within GFF3821 at 91.436 kb on + strand, within GFF3821 at 91.463 kb on + strand, within GFF3821 at 91.479 kb on - strand, within GFF3821 at 91.542 kb on - strand, within GFF3821 at 91.675 kb on + strand at 91.676 kb on - strand at 91.676 kb on - strand at 91.677 kb on + strand at 91.740 kb on + strand, within GFF3822 at 91.781 kb on - strand, within GFF3822 at 91.837 kb on - strand, within GFF3822 at 91.840 kb on + strand, within GFF3822 at 91.951 kb on - strand, within GFF3822 at 91.951 kb on - strand, within GFF3822 at 92.044 kb on - strand, within GFF3822
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 89,402 + GFF3819 0.41 +0.3 89,422 + GFF3819 0.42 +0.1 89,423 - GFF3819 0.42 +0.8 89,537 + GFF3819 0.49 -0.1 89,537 + GFF3819 0.49 +0.0 89,537 + GFF3819 0.49 -0.7 89,685 - GFF3819 0.58 +0.4 89,685 - GFF3819 0.58 +0.4 89,724 - GFF3819 0.60 -0.4 89,784 + GFF3819 0.64 -1.1 89,874 - GFF3819 0.69 +0.4 90,004 + GFF3819 0.77 -0.2 90,134 + GFF3819 0.85 +0.1 90,216 - GFF3819 0.90 -0.4 90,257 + +1.1 90,324 - -1.0 90,360 - -0.4 90,365 - +1.9 90,520 + GFF3820 0.18 -0.0 90,520 + GFF3820 0.18 -0.7 90,724 - GFF3820 0.47 -0.4 90,866 - GFF3820 0.66 -0.4 90,982 + GFF3820 0.82 -1.0 91,205 + GFF3821 0.11 -0.7 91,205 + GFF3821 0.11 -0.1 91,415 + GFF3821 0.56 +0.4 91,415 + GFF3821 0.56 +0.1 91,416 - GFF3821 0.56 -0.2 91,436 + GFF3821 0.60 -0.2 91,463 + GFF3821 0.66 +0.4 91,479 - GFF3821 0.69 -1.2 91,542 - GFF3821 0.83 +0.4 91,675 + -0.4 91,676 - -0.6 91,676 - +0.1 91,677 + -0.8 91,740 + GFF3822 0.14 -0.4 91,781 - GFF3822 0.24 +0.5 91,837 - GFF3822 0.37 +1.5 91,840 + GFF3822 0.38 +0.3 91,951 - GFF3822 0.64 +0.6 91,951 - GFF3822 0.64 -1.1 92,044 - GFF3822 0.86 -1.2
Or see this region's nucleotide sequence