Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF365 and GFF366 are separated by 125 nucleotides GFF366 and GFF367 are separated by 78 nucleotides GFF367 and GFF368 are separated by 77 nucleotides
GFF365 - Protein YicC, at 84,694 to 85,557
GFF365
GFF366 - Ribonuclease PH (EC 2.7.7.56), at 85,683 to 86,399
GFF366
GFF367 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 86,478 to 87,119
GFF367
GFF368 - Transcriptional regulator SlmA, TetR family, at 87,197 to 87,793
GFF368
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-2
-1
0
1 at 84.703 kb on - strand at 84.764 kb on - strand at 84.845 kb on - strand, within GFF365 at 84.845 kb on - strand, within GFF365 at 84.873 kb on - strand, within GFF365 at 85.454 kb on + strand, within GFF365 at 85.454 kb on + strand, within GFF365 at 85.594 kb on - strand at 85.595 kb on + strand at 85.596 kb on - strand at 85.596 kb on - strand at 85.596 kb on - strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.625 kb on - strand at 85.625 kb on - strand at 85.642 kb on - strand at 85.642 kb on - strand at 85.740 kb on + strand at 85.788 kb on - strand, within GFF366 at 85.788 kb on - strand, within GFF366 at 86.035 kb on + strand, within GFF366 at 86.036 kb on - strand, within GFF366 at 86.282 kb on - strand, within GFF366 at 86.488 kb on - strand at 86.546 kb on + strand, within GFF367 at 86.598 kb on - strand, within GFF367 at 86.825 kb on - strand, within GFF367 at 86.868 kb on - strand, within GFF367 at 86.894 kb on + strand, within GFF367 at 86.997 kb on + strand, within GFF367 at 87.005 kb on + strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.151 kb on - strand at 87.151 kb on - strand at 87.290 kb on - strand, within GFF368 at 87.343 kb on - strand, within GFF368 at 87.375 kb on - strand, within GFF368
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 84,703 - +0.5 84,764 - -0.1 84,845 - GFF365 0.17 -0.4 84,845 - GFF365 0.17 -0.2 84,873 - GFF365 0.21 +0.6 85,454 + GFF365 0.88 -0.5 85,454 + GFF365 0.88 +0.4 85,594 - +0.2 85,595 + +0.6 85,596 - -0.0 85,596 - +0.0 85,596 - -0.3 85,600 - +0.6 85,600 - -1.0 85,600 - -0.1 85,625 - +0.9 85,625 - -0.3 85,642 - -0.3 85,642 - -0.2 85,740 + +0.1 85,788 - GFF366 0.15 +0.0 85,788 - GFF366 0.15 -0.4 86,035 + GFF366 0.49 -0.3 86,036 - GFF366 0.49 -1.1 86,282 - GFF366 0.84 +0.1 86,488 - -2.1 86,546 + GFF367 0.11 +0.2 86,598 - GFF367 0.19 +0.2 86,825 - GFF367 0.54 -0.3 86,868 - GFF367 0.61 +1.1 86,894 + GFF367 0.65 -0.5 86,997 + GFF367 0.81 +0.0 87,005 + GFF367 0.82 -0.4 87,006 - GFF367 0.82 +0.4 87,006 - GFF367 0.82 +0.4 87,006 - GFF367 0.82 -0.3 87,006 - GFF367 0.82 -1.9 87,006 - GFF367 0.82 -0.6 87,151 - -0.2 87,151 - -0.1 87,290 - GFF368 0.16 +0.2 87,343 - GFF368 0.24 -0.2 87,375 - GFF368 0.30 +0.2
Or see this region's nucleotide sequence