Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3590 and GFF3591 are separated by 99 nucleotides GFF3591 and GFF3592 are separated by 2 nucleotides GFF3592 and GFF3593 are separated by 57 nucleotides GFF3593 and GFF3594 are separated by 15 nucleotides
GFF3590 - Secretion system effector SseC, at 347,050 to 348,504
GFF3590
GFF3591 - Secretion system effector SseD, at 348,604 to 349,107
GFF3591
GFF3592 - Secretion system effector SseE, at 349,110 to 349,526
GFF3592
GFF3593 - Secretion system chaparone SscB, at 349,584 to 350,012
GFF3593
GFF3594 - Type III secretion effector SseF, at 350,028 to 350,810
GFF3594
Position (kb) 348
349
350 Strain fitness (log2 ratio) -1
0
1 at 347.623 kb on + strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.712 kb on + strand, within GFF3590 at 347.727 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 348.436 kb on - strand at 348.443 kb on + strand at 348.444 kb on - strand at 348.446 kb on + strand at 348.447 kb on - strand at 348.836 kb on + strand, within GFF3591 at 348.948 kb on + strand, within GFF3591 at 349.168 kb on - strand, within GFF3592 at 349.186 kb on + strand, within GFF3592 at 349.190 kb on - strand, within GFF3592 at 349.230 kb on + strand, within GFF3592 at 349.316 kb on - strand, within GFF3592 at 349.336 kb on + strand, within GFF3592 at 349.578 kb on - strand at 349.578 kb on - strand at 350.107 kb on + strand, within GFF3594
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 347,623 + GFF3590 0.39 -0.5 347,624 - GFF3590 0.39 -0.0 347,624 - GFF3590 0.39 -0.6 347,624 - GFF3590 0.39 -0.5 347,712 + GFF3590 0.45 +0.1 347,727 + GFF3590 0.47 +0.5 347,868 + GFF3590 0.56 -0.3 347,868 + GFF3590 0.56 +1.1 348,436 - -0.3 348,443 + +0.5 348,444 - -0.4 348,446 + +0.7 348,447 - -0.8 348,836 + GFF3591 0.46 -0.4 348,948 + GFF3591 0.68 -1.3 349,168 - GFF3592 0.14 +0.0 349,186 + GFF3592 0.18 -0.6 349,190 - GFF3592 0.19 +0.2 349,230 + GFF3592 0.29 +0.3 349,316 - GFF3592 0.49 -0.2 349,336 + GFF3592 0.54 +0.3 349,578 - -0.1 349,578 - +0.5 350,107 + GFF3594 0.10 +0.2
Or see this region's nucleotide sequence