Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF353 and GFF354 are separated by 2 nucleotides GFF354 and GFF355 are separated by 2 nucleotides GFF355 and GFF356 are separated by 5 nucleotides GFF356 and GFF357 are separated by 4 nucleotides
GFF353 - Sodium/glutamate symport protein, at 72,054 to 73,259
GFF353
GFF354 - putative cytoplasmic protein, at 73,262 to 74,113
GFF354
GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197
GFF355
GFF356 - 'tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)' transl_table=11, at 76,203 to 76,892
GFF356
GFF357 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 76,897 to 79,008
GFF357
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -1
0
1
2
3 at 73.118 kb on + strand, within GFF353 at 73.122 kb on - strand, within GFF353 at 73.158 kb on - strand at 73.440 kb on - strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.593 kb on - strand, within GFF354 at 73.814 kb on + strand, within GFF354 at 73.827 kb on + strand, within GFF354 at 73.891 kb on - strand, within GFF354 at 75.192 kb on + strand, within GFF355 at 75.430 kb on + strand, within GFF355 at 75.699 kb on + strand, within GFF355 at 76.128 kb on + strand at 76.418 kb on - strand, within GFF356 at 76.593 kb on - strand, within GFF356 at 76.899 kb on - strand at 76.970 kb on - strand at 76.987 kb on - strand at 77.002 kb on - strand at 77.005 kb on - strand at 77.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 73,118 + GFF353 0.88 +0.1 73,122 - GFF353 0.89 -0.4 73,158 - +0.6 73,440 - GFF354 0.21 -0.6 73,490 + GFF354 0.27 -0.2 73,490 + GFF354 0.27 +0.7 73,593 - GFF354 0.39 +0.2 73,814 + GFF354 0.65 +1.1 73,827 + GFF354 0.66 +3.1 73,891 - GFF354 0.74 +0.3 75,192 + GFF355 0.52 -1.1 75,430 + GFF355 0.63 -1.5 75,699 + GFF355 0.76 -1.7 76,128 + -1.3 76,418 - GFF356 0.31 +0.0 76,593 - GFF356 0.57 -0.2 76,899 - +0.1 76,970 - +0.5 76,987 - +0.8 77,002 - -0.2 77,005 - +0.3 77,062 - +0.3
Or see this region's nucleotide sequence