Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3517 and GFF3518 are separated by 48 nucleotides GFF3518 and GFF3519 are separated by 142 nucleotides GFF3519 and GFF3520 overlap by 4 nucleotides
GFF3517 - Putative outer membrane protein, at 268,397 to 269,122
GFF3517
GFF3518 - putative outer membrane protein, at 269,171 to 270,130
GFF3518
GFF3519 - Putative inner membrane protein, at 270,273 to 270,587
GFF3519
GFF3520 - Nuclease precursor (EC 3.1.30.2), at 270,584 to 271,438
GFF3520
Position (kb) 269
270
271 Strain fitness (log2 ratio) -1
0
1 at 268.358 kb on - strand at 268.359 kb on + strand at 268.427 kb on + strand at 268.589 kb on - strand, within GFF3517 at 268.702 kb on + strand, within GFF3517 at 268.852 kb on + strand, within GFF3517 at 268.853 kb on - strand, within GFF3517 at 268.902 kb on - strand, within GFF3517 at 268.927 kb on + strand, within GFF3517 at 268.928 kb on - strand, within GFF3517 at 268.974 kb on + strand, within GFF3517 at 268.975 kb on - strand, within GFF3517 at 269.040 kb on + strand, within GFF3517 at 269.123 kb on + strand at 269.123 kb on + strand at 269.202 kb on + strand at 269.315 kb on - strand, within GFF3518 at 270.008 kb on - strand, within GFF3518 at 270.381 kb on - strand, within GFF3519 at 270.627 kb on + strand at 270.641 kb on - strand at 270.644 kb on - strand at 270.664 kb on + strand at 270.694 kb on + strand, within GFF3520 at 270.699 kb on + strand, within GFF3520 at 270.718 kb on + strand, within GFF3520 at 270.719 kb on - strand, within GFF3520 at 270.724 kb on + strand, within GFF3520 at 270.776 kb on + strand, within GFF3520 at 270.776 kb on + strand, within GFF3520 at 270.776 kb on + strand, within GFF3520 at 270.777 kb on - strand, within GFF3520 at 270.790 kb on + strand, within GFF3520 at 270.793 kb on + strand, within GFF3520 at 270.935 kb on - strand, within GFF3520
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 268,358 - -0.0 268,359 + +0.4 268,427 + -0.8 268,589 - GFF3517 0.26 +0.6 268,702 + GFF3517 0.42 +0.2 268,852 + GFF3517 0.63 +0.2 268,853 - GFF3517 0.63 +0.3 268,902 - GFF3517 0.70 -0.9 268,927 + GFF3517 0.73 +0.3 268,928 - GFF3517 0.73 +1.1 268,974 + GFF3517 0.79 +0.0 268,975 - GFF3517 0.80 -1.1 269,040 + GFF3517 0.89 +0.2 269,123 + +0.4 269,123 + +0.6 269,202 + +0.4 269,315 - GFF3518 0.15 -1.2 270,008 - GFF3518 0.87 -0.9 270,381 - GFF3519 0.34 -0.0 270,627 + -0.4 270,641 - -0.1 270,644 - -0.5 270,664 + +0.2 270,694 + GFF3520 0.13 -0.2 270,699 + GFF3520 0.13 -1.6 270,718 + GFF3520 0.16 +0.5 270,719 - GFF3520 0.16 -0.2 270,724 + GFF3520 0.16 -0.6 270,776 + GFF3520 0.22 +0.0 270,776 + GFF3520 0.22 -0.0 270,776 + GFF3520 0.22 -0.2 270,777 - GFF3520 0.23 +0.5 270,790 + GFF3520 0.24 -0.1 270,793 + GFF3520 0.24 -0.7 270,935 - GFF3520 0.41 +0.1
Or see this region's nucleotide sequence