Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3479 and GFF3480 are separated by 41 nucleotides GFF3480 and GFF3481 are separated by 351 nucleotides GFF3481 and GFF3482 are separated by 26 nucleotides
GFF3479 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 232,083 to 232,496
GFF3479
GFF3480 - Uncharacterized protein YeaC, at 232,538 to 232,816
GFF3480
GFF3481 - Nicotinamidase (EC 3.5.1.19), at 233,168 to 233,824
GFF3481
GFF3482 - L-asparaginase I, cytoplasmic (EC 3.5.1.1), at 233,851 to 234,867
GFF3482
Position (kb) 233
234 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 232.176 kb on - strand, within GFF3479 at 232.193 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.324 kb on + strand, within GFF3479 at 232.325 kb on - strand, within GFF3479 at 232.439 kb on + strand, within GFF3479 at 232.580 kb on + strand, within GFF3480 at 232.580 kb on + strand, within GFF3480 at 232.612 kb on + strand, within GFF3480 at 232.677 kb on + strand, within GFF3480 at 232.711 kb on + strand, within GFF3480 at 232.712 kb on - strand, within GFF3480 at 232.712 kb on - strand, within GFF3480 at 232.835 kb on + strand at 233.113 kb on + strand at 233.141 kb on + strand at 233.142 kb on - strand at 233.142 kb on - strand at 233.170 kb on - strand at 233.216 kb on - strand at 233.217 kb on + strand at 233.218 kb on - strand at 233.330 kb on + strand, within GFF3481 at 233.361 kb on + strand, within GFF3481 at 233.387 kb on - strand, within GFF3481 at 233.466 kb on + strand, within GFF3481 at 233.467 kb on - strand, within GFF3481 at 233.496 kb on + strand, within GFF3481 at 233.497 kb on - strand, within GFF3481 at 233.497 kb on - strand, within GFF3481 at 233.538 kb on - strand, within GFF3481 at 233.616 kb on + strand, within GFF3481 at 233.808 kb on - strand at 234.143 kb on + strand, within GFF3482 at 234.190 kb on + strand, within GFF3482 at 234.250 kb on - strand, within GFF3482 at 234.263 kb on - strand, within GFF3482 at 234.412 kb on - strand, within GFF3482 at 234.414 kb on + strand, within GFF3482 at 234.427 kb on + strand, within GFF3482 at 234.477 kb on + strand, within GFF3482 at 234.623 kb on + strand, within GFF3482 at 234.658 kb on - strand, within GFF3482 at 234.681 kb on + strand, within GFF3482 at 234.682 kb on - strand, within GFF3482 at 234.795 kb on + strand at 234.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 232,176 - GFF3479 0.22 -0.2 232,193 + GFF3479 0.27 +0.3 232,320 + GFF3479 0.57 -0.2 232,320 + GFF3479 0.57 -0.7 232,324 + GFF3479 0.58 +0.2 232,325 - GFF3479 0.58 -0.1 232,439 + GFF3479 0.86 +0.3 232,580 + GFF3480 0.15 -0.7 232,580 + GFF3480 0.15 -0.1 232,612 + GFF3480 0.27 +0.6 232,677 + GFF3480 0.50 +0.9 232,711 + GFF3480 0.62 +1.1 232,712 - GFF3480 0.62 +0.8 232,712 - GFF3480 0.62 -0.2 232,835 + -0.2 233,113 + -0.5 233,141 + +3.4 233,142 - -0.1 233,142 - +0.2 233,170 - +0.4 233,216 - -2.0 233,217 + +0.7 233,218 - +0.0 233,330 + GFF3481 0.25 +1.2 233,361 + GFF3481 0.29 +0.6 233,387 - GFF3481 0.33 -0.1 233,466 + GFF3481 0.45 -0.2 233,467 - GFF3481 0.46 +0.8 233,496 + GFF3481 0.50 -0.1 233,497 - GFF3481 0.50 +0.9 233,497 - GFF3481 0.50 +0.9 233,538 - GFF3481 0.56 +0.2 233,616 + GFF3481 0.68 +0.0 233,808 - -1.4 234,143 + GFF3482 0.29 -0.3 234,190 + GFF3482 0.33 +0.3 234,250 - GFF3482 0.39 +0.1 234,263 - GFF3482 0.41 -0.9 234,412 - GFF3482 0.55 +1.4 234,414 + GFF3482 0.55 +0.5 234,427 + GFF3482 0.57 -0.4 234,477 + GFF3482 0.62 -0.4 234,623 + GFF3482 0.76 +0.7 234,658 - GFF3482 0.79 +0.2 234,681 + GFF3482 0.82 -0.0 234,682 - GFF3482 0.82 -0.4 234,795 + +0.7 234,805 - -0.6
Or see this region's nucleotide sequence