Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3477

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3476 and GFF3477 are separated by 98 nucleotidesGFF3477 and GFF3478 are separated by 334 nucleotides GFF3476 - Putative oxidoreductase YeaE, aldo/keto reductase family, at 228,580 to 229,428 GFF3476 GFF3477 - Aldose 1-epimerase family protein YeaD, at 229,527 to 230,411 GFF3477 GFF3478 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 230,746 to 231,741 GFF3478 Position (kb) 229 230 231Strain fitness (log2 ratio) -1 0 1 2 3at 228.545 kb on + strandat 228.640 kb on + strandat 228.641 kb on - strandat 228.641 kb on - strandat 228.642 kb on + strandat 228.737 kb on - strand, within GFF3476at 228.776 kb on + strand, within GFF3476at 228.901 kb on + strand, within GFF3476at 228.902 kb on - strand, within GFF3476at 229.505 kb on + strandat 229.547 kb on + strandat 229.812 kb on + strand, within GFF3477at 229.812 kb on + strand, within GFF3477at 230.067 kb on + strand, within GFF3477at 230.068 kb on - strand, within GFF3477at 230.226 kb on - strand, within GFF3477at 230.238 kb on + strand, within GFF3477at 230.286 kb on + strand, within GFF3477at 230.425 kb on + strandat 230.565 kb on - strandat 230.569 kb on - strandat 230.685 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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228,545 + +0.5
228,640 + -0.1
228,641 - -0.2
228,641 - -0.6
228,642 + -0.2
228,737 - GFF3476 0.18 +0.2
228,776 + GFF3476 0.23 +0.8
228,901 + GFF3476 0.38 +0.3
228,902 - GFF3476 0.38 -0.1
229,505 + -0.2
229,547 + +0.2
229,812 + GFF3477 0.32 -1.5
229,812 + GFF3477 0.32 -0.1
230,067 + GFF3477 0.61 -0.3
230,068 - GFF3477 0.61 -1.2
230,226 - GFF3477 0.79 -0.0
230,238 + GFF3477 0.80 -0.0
230,286 + GFF3477 0.86 -0.7
230,425 + +0.3
230,565 - +0.1
230,569 - -0.2
230,685 - +3.1

Or see this region's nucleotide sequence