Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3320

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3317 and GFF3318 are separated by 26 nucleotidesGFF3318 and GFF3319 are separated by 26 nucleotidesGFF3319 and GFF3320 are separated by 4 nucleotidesGFF3320 and GFF3321 are separated by 754 nucleotides GFF3317 - Curli production assembly/transport component CsgG, at 90,076 to 90,909 GFF3317 GFF3318 - Curli production assembly/transport component CsgF, at 90,936 to 91,352 GFF3318 GFF3319 - Curli production assembly/transport component CsgE, at 91,379 to 91,774 GFF3319 GFF3320 - Transcriptional regulator CsgD for 2nd curli operon, at 91,779 to 92,429 GFF3320 GFF3321 - Minor curlin subunit CsgB, nucleation component of curlin monomers, at 93,184 to 93,639 GFF3321 Position (kb) 91 92 93Strain fitness (log2 ratio) -1 0 1at 90.784 kb on + strand, within GFF3317at 90.915 kb on - strandat 92.166 kb on - strand, within GFF3320at 92.392 kb on - strandat 92.392 kb on - strandat 92.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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90,784 + GFF3317 0.85 -0.1
90,915 - +0.3
92,166 - GFF3320 0.59 -0.5
92,392 - -0.1
92,392 - -1.1
92,409 - -0.2

Or see this region's nucleotide sequence