Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3268 and GFF3269 are separated by 361 nucleotides GFF3269 and GFF3270 are separated by 31 nucleotides GFF3270 and GFF3271 are separated by 2 nucleotides GFF3271 and GFF3272 are separated by 79 nucleotides
GFF3268 - Inositol phosphate phosphatase sopB (EC 3.1.3.-), at 40,097 to 41,782
GFF3268
GFF3269 - FIG01045843: hypothetical protein, at 42,144 to 42,284
GFF3269
GFF3270 - FIG01046223: hypothetical protein, at 42,316 to 42,501
GFF3270
GFF3271 - Probable dipeptidase (EC 3.4.-.-), at 42,504 to 44,066
GFF3271
GFF3272 - Putative two component system histidine kinase YedV, at 44,146 to 45,495
GFF3272
Position (kb) 42
43
44
45 Strain fitness (log2 ratio) -1
0
1 at 41.546 kb on + strand, within GFF3268 at 41.553 kb on + strand, within GFF3268 at 41.554 kb on - strand, within GFF3268 at 41.560 kb on + strand, within GFF3268 at 41.635 kb on + strand at 41.783 kb on - strand at 41.944 kb on - strand at 41.944 kb on - strand at 42.064 kb on - strand at 42.275 kb on + strand at 42.294 kb on + strand at 42.336 kb on - strand, within GFF3270 at 42.336 kb on - strand, within GFF3270 at 42.340 kb on - strand, within GFF3270 at 42.705 kb on - strand, within GFF3271 at 42.764 kb on - strand, within GFF3271 at 42.919 kb on + strand, within GFF3271 at 42.920 kb on - strand, within GFF3271 at 43.381 kb on + strand, within GFF3271 at 43.381 kb on + strand, within GFF3271 at 43.381 kb on + strand, within GFF3271 at 43.439 kb on - strand, within GFF3271 at 43.441 kb on - strand, within GFF3271 at 43.632 kb on - strand, within GFF3271 at 43.634 kb on - strand, within GFF3271 at 43.634 kb on - strand, within GFF3271 at 43.707 kb on - strand, within GFF3271 at 43.730 kb on - strand, within GFF3271 at 43.782 kb on - strand, within GFF3271 at 43.823 kb on + strand, within GFF3271 at 43.998 kb on + strand at 44.147 kb on + strand at 44.147 kb on + strand at 44.187 kb on + strand at 44.289 kb on + strand, within GFF3272 at 44.290 kb on - strand, within GFF3272 at 44.290 kb on - strand, within GFF3272 at 44.399 kb on - strand, within GFF3272 at 44.421 kb on - strand, within GFF3272 at 44.601 kb on + strand, within GFF3272 at 44.946 kb on + strand, within GFF3272
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 41,546 + GFF3268 0.86 -0.6 41,553 + GFF3268 0.86 +0.7 41,554 - GFF3268 0.86 +0.7 41,560 + GFF3268 0.87 +0.3 41,635 + +0.0 41,783 - +0.1 41,944 - -1.0 41,944 - +0.4 42,064 - -0.0 42,275 + -0.3 42,294 + -1.3 42,336 - GFF3270 0.11 -0.2 42,336 - GFF3270 0.11 +0.1 42,340 - GFF3270 0.13 +0.1 42,705 - GFF3271 0.13 -1.4 42,764 - GFF3271 0.17 +0.3 42,919 + GFF3271 0.27 +0.2 42,920 - GFF3271 0.27 +0.1 43,381 + GFF3271 0.56 -0.2 43,381 + GFF3271 0.56 +0.6 43,381 + GFF3271 0.56 +0.2 43,439 - GFF3271 0.60 +0.9 43,441 - GFF3271 0.60 -0.2 43,632 - GFF3271 0.72 +0.4 43,634 - GFF3271 0.72 +0.5 43,634 - GFF3271 0.72 +1.4 43,707 - GFF3271 0.77 +0.7 43,730 - GFF3271 0.78 -0.1 43,782 - GFF3271 0.82 +0.6 43,823 + GFF3271 0.84 -0.6 43,998 + -0.4 44,147 + -0.5 44,147 + +0.6 44,187 + -0.7 44,289 + GFF3272 0.11 +0.1 44,290 - GFF3272 0.11 -0.2 44,290 - GFF3272 0.11 -0.4 44,399 - GFF3272 0.19 +0.2 44,421 - GFF3272 0.20 +0.7 44,601 + GFF3272 0.34 -0.1 44,946 + GFF3272 0.59 -0.5
Or see this region's nucleotide sequence