Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3122

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3120 and GFF3121 are separated by 139 nucleotidesGFF3121 and GFF3122 are separated by 63 nucleotidesGFF3122 and GFF3123 overlap by 11 nucleotidesGFF3123 and GFF3124 are separated by 86 nucleotides GFF3120 - Cystathionine beta-lyase (EC 4.4.1.8), at 126,130 to 127,317 GFF3120 GFF3121 - DedA family inner membrane protein YghB, at 127,457 to 128,116 GFF3121 GFF3122 - Transcriptional regulator, AraC family, at 128,180 to 129,079 GFF3122 GFF3123 - hypothetical protein, at 129,069 to 129,185 GFF3123 GFF3124 - NADPH-dependent broad range aldehyde dehydrogenase YqhD, at 129,272 to 130,435 GFF3124 Position (kb) 128 129 130Strain fitness (log2 ratio) -2 -1 0 1 2at 127.316 kb on - strandat 127.336 kb on + strandat 127.337 kb on - strandat 127.337 kb on - strandat 127.338 kb on + strandat 127.338 kb on + strandat 127.338 kb on + strandat 127.338 kb on + strandat 127.339 kb on - strandat 127.339 kb on - strandat 127.339 kb on - strandat 127.339 kb on - strandat 127.339 kb on - strandat 127.339 kb on - strandat 127.412 kb on - strandat 127.417 kb on - strandat 127.432 kb on + strandat 127.475 kb on - strandat 127.542 kb on - strand, within GFF3121at 127.599 kb on + strand, within GFF3121at 127.600 kb on - strand, within GFF3121at 127.715 kb on + strand, within GFF3121at 127.739 kb on - strand, within GFF3121at 127.741 kb on + strand, within GFF3121at 127.742 kb on - strand, within GFF3121at 127.785 kb on - strand, within GFF3121at 127.827 kb on + strand, within GFF3121at 127.827 kb on + strand, within GFF3121at 128.019 kb on + strand, within GFF3121at 128.020 kb on - strand, within GFF3121at 128.052 kb on + strandat 128.108 kb on - strandat 128.120 kb on - strandat 128.120 kb on - strandat 128.182 kb on - strandat 128.182 kb on - strandat 128.246 kb on - strandat 128.246 kb on - strandat 128.470 kb on - strand, within GFF3122at 128.488 kb on + strand, within GFF3122at 128.850 kb on - strand, within GFF3122at 128.976 kb on + strand, within GFF3122at 129.012 kb on - strandat 129.158 kb on + strand, within GFF3123at 129.158 kb on + strand, within GFF3123at 129.288 kb on + strandat 129.288 kb on + strandat 129.377 kb on + strandat 129.378 kb on - strandat 129.378 kb on - strandat 129.433 kb on - strand, within GFF3124at 129.482 kb on + strand, within GFF3124at 129.527 kb on - strand, within GFF3124at 129.599 kb on + strand, within GFF3124at 129.807 kb on - strand, within GFF3124at 129.814 kb on + strand, within GFF3124at 129.814 kb on + strand, within GFF3124at 129.815 kb on - strand, within GFF3124at 129.815 kb on - strand, within GFF3124at 129.815 kb on - strand, within GFF3124at 129.866 kb on - strand, within GFF3124at 129.878 kb on + strand, within GFF3124at 129.879 kb on - strand, within GFF3124at 129.933 kb on + strand, within GFF3124at 129.984 kb on - strand, within GFF3124at 130.015 kb on + strand, within GFF3124at 130.072 kb on + strand, within GFF3124at 130.072 kb on + strand, within GFF3124

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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127,316 - -0.2
127,336 + +0.2
127,337 - -0.2
127,337 - +0.3
127,338 + -0.1
127,338 + +0.3
127,338 + +0.2
127,338 + +0.9
127,339 - -1.4
127,339 - -0.8
127,339 - +0.5
127,339 - +0.3
127,339 - +0.4
127,339 - +0.1
127,412 - +0.2
127,417 - +0.4
127,432 + -2.0
127,475 - -0.2
127,542 - GFF3121 0.13 -0.6
127,599 + GFF3121 0.22 -0.8
127,600 - GFF3121 0.22 +0.1
127,715 + GFF3121 0.39 +1.0
127,739 - GFF3121 0.43 -0.3
127,741 + GFF3121 0.43 +1.1
127,742 - GFF3121 0.43 +0.4
127,785 - GFF3121 0.50 -0.0
127,827 + GFF3121 0.56 +0.6
127,827 + GFF3121 0.56 +1.3
128,019 + GFF3121 0.85 +0.1
128,020 - GFF3121 0.85 -0.2
128,052 + +0.5
128,108 - -0.7
128,120 - +0.1
128,120 - +0.3
128,182 - -0.1
128,182 - -0.9
128,246 - -0.3
128,246 - -0.3
128,470 - GFF3122 0.32 +0.2
128,488 + GFF3122 0.34 +0.3
128,850 - GFF3122 0.74 +0.6
128,976 + GFF3122 0.88 +2.4
129,012 - +0.4
129,158 + GFF3123 0.76 +0.2
129,158 + GFF3123 0.76 +0.6
129,288 + +1.6
129,288 + +0.1
129,377 + +0.3
129,378 - -0.1
129,378 - -0.1
129,433 - GFF3124 0.14 +0.1
129,482 + GFF3124 0.18 -0.2
129,527 - GFF3124 0.22 -0.4
129,599 + GFF3124 0.28 -0.4
129,807 - GFF3124 0.46 +0.1
129,814 + GFF3124 0.47 +0.3
129,814 + GFF3124 0.47 +0.3
129,815 - GFF3124 0.47 -0.0
129,815 - GFF3124 0.47 -0.8
129,815 - GFF3124 0.47 -0.0
129,866 - GFF3124 0.51 -0.4
129,878 + GFF3124 0.52 +1.2
129,879 - GFF3124 0.52 +0.4
129,933 + GFF3124 0.57 -0.2
129,984 - GFF3124 0.61 -0.4
130,015 + GFF3124 0.64 -1.8
130,072 + GFF3124 0.69 +0.1
130,072 + GFF3124 0.69 -0.0

Or see this region's nucleotide sequence