Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2972 and GFF2973 are separated by 192 nucleotides GFF2973 and GFF2974 are separated by 156 nucleotides
GFF2972 - ATPase, AFG1 family, at 55,021 to 56,145
GFF2972
GFF2973 - Putative cytochrome d ubiquinol oxidase subunit III (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit III), at 56,338 to 56,736
GFF2973
GFF2974 - Outer membrane stress sensor protease DegQ, serine protease, at 56,893 to 58,260
GFF2974
Position (kb) 56
57 Strain fitness (log2 ratio) -1
0
1 at 55.477 kb on + strand, within GFF2972 at 55.477 kb on + strand, within GFF2972 at 55.533 kb on + strand, within GFF2972 at 55.534 kb on - strand, within GFF2972 at 55.534 kb on - strand, within GFF2972 at 55.552 kb on - strand, within GFF2972 at 55.730 kb on - strand, within GFF2972 at 55.761 kb on + strand, within GFF2972 at 55.872 kb on - strand, within GFF2972 at 55.872 kb on - strand, within GFF2972 at 55.920 kb on + strand, within GFF2972 at 55.920 kb on + strand, within GFF2972 at 55.921 kb on - strand, within GFF2972 at 55.921 kb on - strand, within GFF2972 at 55.921 kb on - strand, within GFF2972 at 55.921 kb on - strand, within GFF2972 at 55.921 kb on - strand, within GFF2972 at 55.922 kb on + strand, within GFF2972 at 55.922 kb on + strand, within GFF2972 at 55.922 kb on + strand, within GFF2972 at 55.923 kb on - strand, within GFF2972 at 55.923 kb on - strand, within GFF2972 at 56.039 kb on + strand at 56.075 kb on - strand at 56.146 kb on - strand at 56.157 kb on + strand at 56.212 kb on + strand at 56.212 kb on + strand at 56.224 kb on + strand at 56.271 kb on + strand at 56.318 kb on + strand at 56.318 kb on + strand at 56.318 kb on + strand at 56.351 kb on - strand at 56.357 kb on + strand at 56.415 kb on + strand, within GFF2973 at 56.415 kb on + strand, within GFF2973 at 56.415 kb on + strand, within GFF2973 at 56.416 kb on - strand, within GFF2973 at 56.480 kb on - strand, within GFF2973 at 56.712 kb on - strand at 56.808 kb on - strand at 56.808 kb on - strand at 56.817 kb on - strand at 56.877 kb on + strand at 56.916 kb on - strand at 56.916 kb on - strand at 56.930 kb on + strand at 56.931 kb on - strand at 57.025 kb on - strand at 57.199 kb on + strand, within GFF2974 at 57.199 kb on + strand, within GFF2974 at 57.203 kb on + strand, within GFF2974 at 57.203 kb on + strand, within GFF2974 at 57.204 kb on - strand, within GFF2974 at 57.204 kb on - strand, within GFF2974 at 57.204 kb on - strand, within GFF2974 at 57.213 kb on + strand, within GFF2974 at 57.226 kb on - strand, within GFF2974 at 57.628 kb on - strand, within GFF2974 at 57.706 kb on - strand, within GFF2974
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 55,477 + GFF2972 0.41 -0.1 55,477 + GFF2972 0.41 -0.2 55,533 + GFF2972 0.46 +0.3 55,534 - GFF2972 0.46 +0.0 55,534 - GFF2972 0.46 +0.3 55,552 - GFF2972 0.47 +0.5 55,730 - GFF2972 0.63 -0.2 55,761 + GFF2972 0.66 +0.2 55,872 - GFF2972 0.76 +1.7 55,872 - GFF2972 0.76 +0.2 55,920 + GFF2972 0.80 +0.1 55,920 + GFF2972 0.80 +0.5 55,921 - GFF2972 0.80 +0.3 55,921 - GFF2972 0.80 +0.9 55,921 - GFF2972 0.80 -0.6 55,921 - GFF2972 0.80 +0.0 55,921 - GFF2972 0.80 +0.1 55,922 + GFF2972 0.80 -0.1 55,922 + GFF2972 0.80 -0.1 55,922 + GFF2972 0.80 +0.1 55,923 - GFF2972 0.80 +0.2 55,923 - GFF2972 0.80 -0.5 56,039 + +0.1 56,075 - -0.8 56,146 - +0.6 56,157 + +0.4 56,212 + +0.5 56,212 + -0.1 56,224 + +0.1 56,271 + -0.1 56,318 + -0.1 56,318 + +0.2 56,318 + -1.2 56,351 - -0.3 56,357 + +0.2 56,415 + GFF2973 0.19 -0.7 56,415 + GFF2973 0.19 +0.4 56,415 + GFF2973 0.19 -1.1 56,416 - GFF2973 0.20 -0.1 56,480 - GFF2973 0.36 -0.5 56,712 - +1.0 56,808 - +0.8 56,808 - +0.2 56,817 - +0.3 56,877 + +0.0 56,916 - +1.4 56,916 - +0.2 56,930 + -0.1 56,931 - +0.2 57,025 - +0.5 57,199 + GFF2974 0.22 +0.4 57,199 + GFF2974 0.22 -0.4 57,203 + GFF2974 0.23 -0.2 57,203 + GFF2974 0.23 -0.8 57,204 - GFF2974 0.23 -0.6 57,204 - GFF2974 0.23 -0.2 57,204 - GFF2974 0.23 -0.1 57,213 + GFF2974 0.23 +0.3 57,226 - GFF2974 0.24 +0.3 57,628 - GFF2974 0.54 -1.1 57,706 - GFF2974 0.59 -0.1
Or see this region's nucleotide sequence