Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2895

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2894 and GFF2895 are separated by 537 nucleotidesGFF2895 and GFF2896 are separated by 91 nucleotides GFF2894 - ATP-dependent DNA helicase Rep, at 19,403 to 21,427 GFF2894 GFF2895 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 21,965 to 22,246 GFF2895 GFF2896 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 22,338 to 23,813 GFF2896 Position (kb) 21 22 23Strain fitness (log2 ratio) -1 0 1at 21.466 kb on - strandat 21.554 kb on - strandat 21.554 kb on - strandat 21.624 kb on + strandat 21.722 kb on - strandat 21.847 kb on + strandat 21.900 kb on + strandat 21.900 kb on + strandat 21.900 kb on + strandat 21.900 kb on + strandat 21.900 kb on + strandat 21.900 kb on + strandat 21.901 kb on - strandat 21.902 kb on + strandat 21.903 kb on - strandat 21.992 kb on - strandat 22.158 kb on + strand, within GFF2895at 22.334 kb on + strandat 22.336 kb on + strandat 22.337 kb on - strandat 22.337 kb on - strandat 22.392 kb on - strandat 22.396 kb on - strandat 22.396 kb on - strandat 22.415 kb on + strandat 22.549 kb on + strand, within GFF2896at 22.559 kb on - strand, within GFF2896at 22.578 kb on - strand, within GFF2896at 22.652 kb on - strand, within GFF2896at 22.688 kb on + strand, within GFF2896at 22.689 kb on - strand, within GFF2896at 22.754 kb on - strand, within GFF2896

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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21,466 - +0.1
21,554 - -0.1
21,554 - -0.3
21,624 + -0.3
21,722 - +0.6
21,847 + -0.1
21,900 + +0.1
21,900 + -0.2
21,900 + +0.8
21,900 + +0.7
21,900 + -0.2
21,900 + +0.4
21,901 - -0.2
21,902 + -0.0
21,903 - -0.5
21,992 - +0.0
22,158 + GFF2895 0.68 -0.6
22,334 + +0.3
22,336 + +0.2
22,337 - -0.5
22,337 - +0.7
22,392 - -0.3
22,396 - -0.2
22,396 - +0.3
22,415 + +1.3
22,549 + GFF2896 0.14 -0.8
22,559 - GFF2896 0.15 -0.5
22,578 - GFF2896 0.16 +0.3
22,652 - GFF2896 0.21 +0.6
22,688 + GFF2896 0.24 +0.2
22,689 - GFF2896 0.24 +0.6
22,754 - GFF2896 0.28 +1.0

Or see this region's nucleotide sequence