Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2880 and GFF2881 overlap by 4 nucleotides GFF2881 and GFF2882 are separated by 1 nucleotides
GFF2880 - 4-alpha-L-fucosyltransferase (EC 2.4.1.-), at 4,069 to 5,148
GFF2880
GFF2881 - WzxE protein, at 5,145 to 6,395
GFF2881
GFF2882 - Lipopolysaccharide biosynthesis protein RffA, at 6,397 to 7,527
GFF2882
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4.156 kb on + strand at 4.200 kb on + strand, within GFF2880 at 4.276 kb on + strand, within GFF2880 at 4.464 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.533 kb on - strand, within GFF2880 at 4.827 kb on + strand, within GFF2880 at 4.969 kb on - strand, within GFF2880 at 5.103 kb on + strand at 5.201 kb on + strand at 5.202 kb on - strand at 5.203 kb on + strand at 5.203 kb on + strand at 5.213 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.216 kb on - strand at 5.382 kb on + strand, within GFF2881 at 5.605 kb on + strand, within GFF2881 at 5.605 kb on + strand, within GFF2881 at 5.618 kb on + strand, within GFF2881 at 5.625 kb on + strand, within GFF2881 at 5.657 kb on + strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.659 kb on + strand, within GFF2881 at 5.692 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.820 kb on + strand, within GFF2881 at 5.822 kb on + strand, within GFF2881 at 5.836 kb on - strand, within GFF2881 at 5.946 kb on + strand, within GFF2881 at 6.052 kb on + strand, within GFF2881 at 6.080 kb on + strand, within GFF2881 at 6.122 kb on + strand, within GFF2881 at 6.123 kb on - strand, within GFF2881 at 6.135 kb on + strand, within GFF2881 at 6.168 kb on + strand, within GFF2881 at 6.169 kb on - strand, within GFF2881 at 6.182 kb on + strand, within GFF2881 at 6.223 kb on - strand, within GFF2881 at 6.395 kb on + strand at 6.421 kb on - strand at 6.458 kb on + strand at 6.459 kb on - strand at 6.504 kb on - strand at 6.567 kb on - strand, within GFF2882 at 6.607 kb on - strand, within GFF2882 at 6.654 kb on + strand, within GFF2882 at 6.655 kb on - strand, within GFF2882 at 6.772 kb on - strand, within GFF2882 at 6.857 kb on + strand, within GFF2882 at 6.858 kb on - strand, within GFF2882 at 6.861 kb on + strand, within GFF2882 at 6.936 kb on + strand, within GFF2882 at 7.002 kb on + strand, within GFF2882 at 7.002 kb on + strand, within GFF2882 at 7.047 kb on - strand, within GFF2882 at 7.092 kb on + strand, within GFF2882 at 7.093 kb on - strand, within GFF2882 at 7.094 kb on + strand, within GFF2882 at 7.094 kb on + strand, within GFF2882 at 7.150 kb on + strand, within GFF2882 at 7.150 kb on + strand, within GFF2882 at 7.162 kb on - strand, within GFF2882 at 7.209 kb on + strand, within GFF2882 at 7.209 kb on + strand, within GFF2882 at 7.290 kb on + strand, within GFF2882
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 4,156 + -0.8 4,200 + GFF2880 0.12 +0.2 4,276 + GFF2880 0.19 +0.2 4,464 + GFF2880 0.37 +0.5 4,466 + GFF2880 0.37 +0.5 4,466 + GFF2880 0.37 +0.2 4,466 + GFF2880 0.37 -0.3 4,466 + GFF2880 0.37 +0.2 4,466 + GFF2880 0.37 +0.2 4,466 + GFF2880 0.37 -1.0 4,533 - GFF2880 0.43 -0.0 4,827 + GFF2880 0.70 -0.4 4,969 - GFF2880 0.83 +0.4 5,103 + +1.5 5,201 + +0.5 5,202 - +1.4 5,203 + +0.5 5,203 + -0.6 5,213 + -0.7 5,215 + -0.2 5,215 + -0.6 5,215 + -0.5 5,215 + -2.0 5,216 - -0.4 5,382 + GFF2881 0.19 -0.6 5,605 + GFF2881 0.37 +0.1 5,605 + GFF2881 0.37 -0.0 5,618 + GFF2881 0.38 -0.0 5,625 + GFF2881 0.38 -0.6 5,657 + GFF2881 0.41 -0.0 5,658 - GFF2881 0.41 -0.9 5,658 - GFF2881 0.41 -0.2 5,659 + GFF2881 0.41 -0.3 5,692 + GFF2881 0.44 +0.2 5,817 + GFF2881 0.54 -1.8 5,817 + GFF2881 0.54 -0.5 5,817 + GFF2881 0.54 +0.1 5,818 - GFF2881 0.54 -0.8 5,818 - GFF2881 0.54 +0.4 5,820 + GFF2881 0.54 -0.8 5,822 + GFF2881 0.54 -0.9 5,836 - GFF2881 0.55 +0.0 5,946 + GFF2881 0.64 -2.0 6,052 + GFF2881 0.73 -1.8 6,080 + GFF2881 0.75 -0.5 6,122 + GFF2881 0.78 -0.3 6,123 - GFF2881 0.78 -1.8 6,135 + GFF2881 0.79 -0.0 6,168 + GFF2881 0.82 -1.8 6,169 - GFF2881 0.82 -0.5 6,182 + GFF2881 0.83 -0.2 6,223 - GFF2881 0.86 -3.0 6,395 + +0.5 6,421 - -0.6 6,458 + +0.4 6,459 - +0.5 6,504 - -2.0 6,567 - GFF2882 0.15 +0.6 6,607 - GFF2882 0.19 +0.4 6,654 + GFF2882 0.23 +0.1 6,655 - GFF2882 0.23 +0.1 6,772 - GFF2882 0.33 -1.6 6,857 + GFF2882 0.41 -0.3 6,858 - GFF2882 0.41 +0.2 6,861 + GFF2882 0.41 +0.5 6,936 + GFF2882 0.48 +1.8 7,002 + GFF2882 0.53 +0.8 7,002 + GFF2882 0.53 +0.2 7,047 - GFF2882 0.57 -0.3 7,092 + GFF2882 0.61 +0.1 7,093 - GFF2882 0.62 -0.8 7,094 + GFF2882 0.62 -0.0 7,094 + GFF2882 0.62 -0.5 7,150 + GFF2882 0.67 +0.1 7,150 + GFF2882 0.67 -0.9 7,162 - GFF2882 0.68 +0.6 7,209 + GFF2882 0.72 +0.2 7,209 + GFF2882 0.72 -0.4 7,290 + GFF2882 0.79 -1.0
Or see this region's nucleotide sequence