Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2653 and GFF2654 overlap by 20 nucleotides GFF2654 and GFF2655 are separated by 257 nucleotides GFF2655 and GFF2656 are separated by 195 nucleotides
GFF2653 - FIG01045924: hypothetical protein, at 178,300 to 178,479
GFF2653
GFF2654 - FIG00638524: hypothetical protein, at 178,460 to 179,023
GFF2654
GFF2655 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 179,281 to 179,796
GFF2655
GFF2656 - Fumarate and nitrate reduction regulatory protein, at 179,992 to 180,744
GFF2656
Position (kb) 179
180 Strain fitness (log2 ratio) -1
0
1 at 178.395 kb on + strand, within GFF2653 at 178.396 kb on - strand, within GFF2653 at 178.468 kb on + strand at 178.516 kb on + strand at 178.531 kb on - strand, within GFF2654 at 178.580 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.678 kb on + strand, within GFF2654 at 178.861 kb on + strand, within GFF2654 at 178.888 kb on + strand, within GFF2654 at 178.949 kb on + strand, within GFF2654 at 179.163 kb on - strand at 179.170 kb on + strand at 179.239 kb on - strand at 179.239 kb on - strand at 179.250 kb on + strand at 179.255 kb on + strand at 179.392 kb on - strand, within GFF2655 at 179.991 kb on + strand at 180.196 kb on + strand, within GFF2656 at 180.226 kb on + strand, within GFF2656 at 180.258 kb on + strand, within GFF2656 at 180.272 kb on + strand, within GFF2656 at 180.676 kb on - strand at 180.679 kb on + strand at 180.679 kb on + strand at 180.680 kb on - strand at 180.681 kb on + strand at 180.684 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 178,395 + GFF2653 0.53 +0.9 178,396 - GFF2653 0.53 +0.0 178,468 + +0.1 178,516 + -0.6 178,531 - GFF2654 0.13 +0.4 178,580 + GFF2654 0.21 +0.6 178,614 + GFF2654 0.27 -0.4 178,614 + GFF2654 0.27 -0.3 178,678 + GFF2654 0.39 +0.2 178,861 + GFF2654 0.71 +0.9 178,888 + GFF2654 0.76 +0.4 178,949 + GFF2654 0.87 -0.2 179,163 - +0.2 179,170 + +0.1 179,239 - +1.3 179,239 - -0.5 179,250 + +0.6 179,255 + +1.7 179,392 - GFF2655 0.22 -0.7 179,991 + -0.3 180,196 + GFF2656 0.27 +0.5 180,226 + GFF2656 0.31 +0.3 180,258 + GFF2656 0.35 +0.7 180,272 + GFF2656 0.37 +0.3 180,676 - +0.9 180,679 + -0.9 180,679 + -1.6 180,680 - -0.6 180,681 + +0.1 180,684 + +0.3
Or see this region's nucleotide sequence