Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2522 and GFF2523 are separated by 5 nucleotides GFF2523 and GFF2524 are separated by 19 nucleotides GFF2524 and GFF2525 are separated by 2 nucleotides
GFF2522 - Hydrogenase maturation protease (EC 3.4.24.-), at 36,305 to 36,913
GFF2522
GFF2523 - Ni,Fe-hydrogenase I cytochrome b subunit, at 36,919 to 37,593
GFF2523
GFF2524 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 37,613 to 39,415
GFF2524
GFF2525 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 39,418 to 40,410
GFF2525
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -2
-1
0
1
2 at 36.660 kb on + strand, within GFF2522 at 36.976 kb on - strand at 36.996 kb on + strand, within GFF2523 at 36.997 kb on - strand, within GFF2523 at 37.143 kb on - strand, within GFF2523 at 37.226 kb on + strand, within GFF2523 at 37.227 kb on - strand, within GFF2523 at 37.227 kb on - strand, within GFF2523 at 37.230 kb on - strand, within GFF2523 at 37.491 kb on + strand, within GFF2523 at 37.554 kb on - strand at 37.557 kb on + strand at 37.557 kb on + strand at 37.558 kb on - strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.560 kb on - strand at 37.657 kb on + strand at 37.830 kb on + strand, within GFF2524 at 37.831 kb on - strand, within GFF2524 at 37.831 kb on - strand, within GFF2524 at 38.053 kb on + strand, within GFF2524 at 38.054 kb on - strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.355 kb on + strand, within GFF2524 at 38.440 kb on + strand, within GFF2524 at 38.860 kb on - strand, within GFF2524 at 38.860 kb on - strand, within GFF2524 at 39.074 kb on + strand, within GFF2524 at 39.079 kb on + strand, within GFF2524 at 39.091 kb on + strand, within GFF2524 at 39.296 kb on - strand at 39.296 kb on - strand at 39.608 kb on + strand, within GFF2525 at 39.705 kb on + strand, within GFF2525 at 39.738 kb on - strand, within GFF2525 at 39.903 kb on - strand, within GFF2525 at 39.903 kb on - strand, within GFF2525 at 39.999 kb on + strand, within GFF2525 at 40.188 kb on - strand, within GFF2525 at 40.357 kb on + strand at 40.378 kb on - strand at 40.392 kb on - strand at 40.397 kb on - strand at 40.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 36,660 + GFF2522 0.58 -0.5 36,976 - -0.2 36,996 + GFF2523 0.11 -1.0 36,997 - GFF2523 0.12 -2.5 37,143 - GFF2523 0.33 -0.5 37,226 + GFF2523 0.45 +0.5 37,227 - GFF2523 0.46 +0.5 37,227 - GFF2523 0.46 +0.8 37,230 - GFF2523 0.46 +0.5 37,491 + GFF2523 0.85 +0.1 37,554 - +0.3 37,557 + -0.7 37,557 + -0.3 37,558 - +0.1 37,559 + -0.1 37,559 + +0.5 37,559 + +0.2 37,559 + +0.0 37,560 - +0.1 37,657 + +0.0 37,830 + GFF2524 0.12 +0.0 37,831 - GFF2524 0.12 +0.8 37,831 - GFF2524 0.12 +0.5 38,053 + GFF2524 0.24 +0.2 38,054 - GFF2524 0.24 -0.1 38,349 + GFF2524 0.41 +0.3 38,349 + GFF2524 0.41 +0.5 38,349 + GFF2524 0.41 +0.6 38,349 + GFF2524 0.41 -0.1 38,355 + GFF2524 0.41 -0.8 38,440 + GFF2524 0.46 +0.2 38,860 - GFF2524 0.69 +1.0 38,860 - GFF2524 0.69 +0.5 39,074 + GFF2524 0.81 -0.1 39,079 + GFF2524 0.81 -0.2 39,091 + GFF2524 0.82 +0.1 39,296 - +0.0 39,296 - -0.1 39,608 + GFF2525 0.19 +1.0 39,705 + GFF2525 0.29 +0.3 39,738 - GFF2525 0.32 +0.5 39,903 - GFF2525 0.49 -0.5 39,903 - GFF2525 0.49 +0.1 39,999 + GFF2525 0.59 +0.1 40,188 - GFF2525 0.78 +0.8 40,357 + -0.2 40,378 - +0.9 40,392 - -0.9 40,397 - +2.5 40,397 - +0.1
Or see this region's nucleotide sequence