Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2509 and GFF2510 are separated by 115 nucleotides GFF2510 and GFF2511 are separated by 102 nucleotides
GFF2509 - Sugar efflux transporter SotB, at 24,519 to 25,709
GFF2509
GFF2510 - LysR family transcriptional regulator YneJ, at 25,825 to 26,697
GFF2510
GFF2511 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 26,800 to 28,188
GFF2511
Position (kb)
25
26
27 Strain fitness (log2 ratio)
-1
0
1
2 at 24.902 kb on + strand, within GFF2509 at 25.018 kb on + strand, within GFF2509 at 25.018 kb on + strand, within GFF2509 at 25.018 kb on + strand, within GFF2509 at 25.018 kb on + strand, within GFF2509 at 25.022 kb on + strand, within GFF2509 at 25.022 kb on + strand, within GFF2509 at 25.022 kb on + strand, within GFF2509 at 25.023 kb on - strand, within GFF2509 at 25.024 kb on + strand, within GFF2509 at 25.025 kb on - strand, within GFF2509 at 25.067 kb on - strand, within GFF2509 at 25.067 kb on - strand, within GFF2509 at 25.146 kb on + strand, within GFF2509 at 25.165 kb on - strand, within GFF2509 at 25.165 kb on - strand, within GFF2509 at 25.165 kb on - strand, within GFF2509 at 25.172 kb on + strand, within GFF2509 at 25.172 kb on + strand, within GFF2509 at 25.243 kb on - strand, within GFF2509 at 25.293 kb on + strand, within GFF2509 at 25.293 kb on + strand, within GFF2509 at 25.294 kb on - strand, within GFF2509 at 25.661 kb on + strand at 25.662 kb on - strand at 25.753 kb on + strand at 25.855 kb on + strand at 25.986 kb on + strand, within GFF2510 at 25.987 kb on - strand, within GFF2510 at 25.987 kb on - strand, within GFF2510 at 26.148 kb on + strand, within GFF2510 at 26.151 kb on - strand, within GFF2510 at 26.366 kb on + strand, within GFF2510 at 26.367 kb on - strand, within GFF2510 at 26.389 kb on - strand, within GFF2510 at 26.498 kb on + strand, within GFF2510 at 26.644 kb on - strand at 26.670 kb on + strand at 27.097 kb on + strand, within GFF2511 at 27.135 kb on + strand, within GFF2511 at 27.264 kb on + strand, within GFF2511 at 27.356 kb on - strand, within GFF2511 at 27.356 kb on - strand, within GFF2511 at 27.372 kb on + strand, within GFF2511 at 27.494 kb on + strand, within GFF2511 at 27.495 kb on - strand, within GFF2511 at 27.522 kb on + strand, within GFF2511
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 24,902 + GFF2509 0.32 +0.4 25,018 + GFF2509 0.42 -0.1 25,018 + GFF2509 0.42 +0.1 25,018 + GFF2509 0.42 -0.5 25,018 + GFF2509 0.42 +0.7 25,022 + GFF2509 0.42 -1.5 25,022 + GFF2509 0.42 -1.7 25,022 + GFF2509 0.42 +0.0 25,023 - GFF2509 0.42 -1.0 25,024 + GFF2509 0.42 +0.2 25,025 - GFF2509 0.42 +0.3 25,067 - GFF2509 0.46 -0.2 25,067 - GFF2509 0.46 -0.9 25,146 + GFF2509 0.53 +0.5 25,165 - GFF2509 0.54 +0.3 25,165 - GFF2509 0.54 +0.6 25,165 - GFF2509 0.54 +0.2 25,172 + GFF2509 0.55 +0.0 25,172 + GFF2509 0.55 -0.7 25,243 - GFF2509 0.61 +0.5 25,293 + GFF2509 0.65 +0.3 25,293 + GFF2509 0.65 -0.1 25,294 - GFF2509 0.65 -0.4 25,661 + -0.1 25,662 - -1.7 25,753 + -0.5 25,855 + -1.1 25,986 + GFF2510 0.18 -0.6 25,987 - GFF2510 0.19 +0.5 25,987 - GFF2510 0.19 +2.0 26,148 + GFF2510 0.37 +0.5 26,151 - GFF2510 0.37 +0.1 26,366 + GFF2510 0.62 +1.7 26,367 - GFF2510 0.62 +0.6 26,389 - GFF2510 0.65 -0.0 26,498 + GFF2510 0.77 -0.3 26,644 - -0.1 26,670 + -0.1 27,097 + GFF2511 0.21 +0.5 27,135 + GFF2511 0.24 +0.3 27,264 + GFF2511 0.33 -0.3 27,356 - GFF2511 0.40 -0.5 27,356 - GFF2511 0.40 -0.4 27,372 + GFF2511 0.41 -1.1 27,494 + GFF2511 0.50 -0.3 27,495 - GFF2511 0.50 -0.1 27,522 + GFF2511 0.52 +0.3
Or see this region's nucleotide sequence