Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF238

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF236 and GFF237 are separated by 36 nucleotidesGFF237 and GFF238 overlap by 4 nucleotidesGFF238 and GFF239 overlap by 4 nucleotidesGFF239 and GFF240 overlap by 1 nucleotides GFF236 - FIG00545237: hypothetical protein, at 10,856 to 11,059 GFF236 GFF237 - Diaminopimelate epimerase (EC 5.1.1.7), at 11,096 to 11,920 GFF237 GFF238 - Protein of unknown function DUF484, at 11,917 to 12,624 GFF238 GFF239 - Tyrosine recombinase XerC, at 12,621 to 13,523 GFF239 GFF240 - 'Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase' transl_table=11, at 13,523 to 14,239 GFF240 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1at 10.935 kb on - strand, within GFF236at 11.054 kb on + strandat 11.917 kb on - strandat 12.129 kb on + strand, within GFF238at 12.330 kb on + strand, within GFF238at 12.330 kb on + strand, within GFF238at 12.620 kb on + strandat 13.346 kb on + strand, within GFF239at 13.550 kb on - strandat 13.586 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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10,935 - GFF236 0.39 -1.2
11,054 + +0.1
11,917 - -1.5
12,129 + GFF238 0.30 +1.0
12,330 + GFF238 0.58 +0.4
12,330 + GFF238 0.58 +0.8
12,620 + -1.1
13,346 + GFF239 0.80 -1.7
13,550 - -0.0
13,586 + +0.2

Or see this region's nucleotide sequence