Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2264 and GFF2265 overlap by 4 nucleotides GFF2265 and GFF2266 are separated by 287 nucleotides
GFF2264 - L-rhamnose isomerase (EC 5.3.1.14), at 54,976 to 56,235
GFF2264
GFF2265 - Rhamnulokinase (EC 2.7.1.5), at 56,232 to 57,701
GFF2265
GFF2266 - L-rhamnose operon regulatory protein RhaS, at 57,989 to 58,825
GFF2266
Position (kb) 56
57
58 Strain fitness (log2 ratio) -1
0
1 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.502 kb on + strand, within GFF2264 at 55.527 kb on - strand, within GFF2264 at 55.600 kb on - strand, within GFF2264 at 55.943 kb on + strand, within GFF2264 at 56.175 kb on + strand at 56.175 kb on + strand at 56.176 kb on - strand at 56.176 kb on - strand at 56.180 kb on - strand at 56.314 kb on + strand at 56.314 kb on + strand at 56.315 kb on - strand at 56.333 kb on + strand at 56.334 kb on - strand at 56.370 kb on + strand at 56.388 kb on - strand, within GFF2265 at 56.397 kb on - strand, within GFF2265 at 56.403 kb on - strand, within GFF2265 at 56.403 kb on - strand, within GFF2265 at 56.567 kb on - strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.942 kb on - strand, within GFF2265 at 56.954 kb on + strand, within GFF2265 at 57.215 kb on - strand, within GFF2265 at 57.283 kb on - strand, within GFF2265 at 57.289 kb on + strand, within GFF2265 at 57.290 kb on - strand, within GFF2265 at 57.445 kb on + strand, within GFF2265 at 57.446 kb on - strand, within GFF2265 at 57.446 kb on - strand, within GFF2265 at 57.634 kb on + strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.736 kb on + strand at 57.757 kb on - strand at 57.834 kb on + strand at 57.837 kb on + strand at 57.948 kb on + strand at 57.948 kb on + strand at 58.004 kb on - strand at 58.135 kb on - strand, within GFF2266 at 58.135 kb on - strand, within GFF2266 at 58.151 kb on + strand, within GFF2266 at 58.153 kb on + strand, within GFF2266 at 58.203 kb on + strand, within GFF2266 at 58.203 kb on + strand, within GFF2266 at 58.213 kb on + strand, within GFF2266 at 58.224 kb on + strand, within GFF2266 at 58.224 kb on + strand, within GFF2266 at 58.225 kb on - strand, within GFF2266 at 58.239 kb on + strand, within GFF2266 at 58.313 kb on + strand, within GFF2266 at 58.317 kb on - strand, within GFF2266 at 58.439 kb on - strand, within GFF2266 at 58.446 kb on + strand, within GFF2266 at 58.700 kb on - strand, within GFF2266
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 55,400 + GFF2264 0.34 +0.4 55,400 + GFF2264 0.34 +0.4 55,400 + GFF2264 0.34 -0.2 55,502 + GFF2264 0.42 +0.3 55,527 - GFF2264 0.44 +0.1 55,600 - GFF2264 0.50 -0.0 55,943 + GFF2264 0.77 -0.4 56,175 + +0.6 56,175 + +0.2 56,176 - +1.4 56,176 - -0.0 56,180 - -1.4 56,314 + +0.2 56,314 + -0.2 56,315 - -0.0 56,333 + -0.5 56,334 - -0.6 56,370 + +0.3 56,388 - GFF2265 0.11 +0.0 56,397 - GFF2265 0.11 +0.0 56,403 - GFF2265 0.12 +0.3 56,403 - GFF2265 0.12 +0.3 56,567 - GFF2265 0.23 +0.0 56,941 + GFF2265 0.48 +0.2 56,941 + GFF2265 0.48 -0.0 56,941 + GFF2265 0.48 -0.5 56,942 - GFF2265 0.48 -0.5 56,954 + GFF2265 0.49 +0.4 57,215 - GFF2265 0.67 -0.1 57,283 - GFF2265 0.71 +0.2 57,289 + GFF2265 0.72 +1.0 57,290 - GFF2265 0.72 +0.5 57,445 + GFF2265 0.83 +0.1 57,446 - GFF2265 0.83 +1.7 57,446 - GFF2265 0.83 -0.0 57,634 + -1.0 57,717 - +0.3 57,717 - +0.2 57,736 + -0.0 57,757 - +0.2 57,834 + -0.9 57,837 + +0.3 57,948 + +0.2 57,948 + +0.4 58,004 - +0.7 58,135 - GFF2266 0.17 +1.6 58,135 - GFF2266 0.17 -0.1 58,151 + GFF2266 0.19 +0.2 58,153 + GFF2266 0.20 -0.9 58,203 + GFF2266 0.26 -0.6 58,203 + GFF2266 0.26 -0.8 58,213 + GFF2266 0.27 -0.1 58,224 + GFF2266 0.28 +0.4 58,224 + GFF2266 0.28 +0.4 58,225 - GFF2266 0.28 -0.7 58,239 + GFF2266 0.30 -0.1 58,313 + GFF2266 0.39 +0.2 58,317 - GFF2266 0.39 -0.2 58,439 - GFF2266 0.54 +0.1 58,446 + GFF2266 0.55 -0.7 58,700 - GFF2266 0.85 -0.5
Or see this region's nucleotide sequence