Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF217 and GFF218 overlap by 23 nucleotides GFF218 and GFF219 are separated by 17 nucleotides GFF219 and GFF220 are separated by 10 nucleotides GFF220 and GFF221 overlap by 4 nucleotides
GFF217 - '2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme- A synthase (EC 2.7.8.25)' transl_table=11, at 215,253 to 216,161
GFF217
GFF218 - Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.61), at 216,139 to 216,672
GFF218
GFF219 - Citrate lyase alpha chain (EC 4.1.3.6), at 216,690 to 218,210
GFF219
GFF220 - Citrate lyase beta chain (EC 4.1.3.6), at 218,221 to 219,090
GFF220
GFF221 - Citrate lyase beta chain (EC 4.1.3.6), at 219,087 to 219,380
GFF221
Position (kb) 216
217
218
219 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 215.855 kb on + strand, within GFF217 at 215.940 kb on - strand, within GFF217 at 215.979 kb on - strand, within GFF217 at 216.009 kb on - strand, within GFF217 at 216.023 kb on + strand, within GFF217 at 216.095 kb on + strand at 216.132 kb on - strand at 216.250 kb on + strand, within GFF218 at 216.300 kb on + strand, within GFF218 at 216.300 kb on + strand, within GFF218 at 216.310 kb on - strand, within GFF218 at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.996 kb on - strand, within GFF219 at 216.999 kb on - strand, within GFF219 at 217.528 kb on - strand, within GFF219 at 217.528 kb on - strand, within GFF219 at 217.633 kb on + strand, within GFF219 at 217.890 kb on - strand, within GFF219 at 217.923 kb on + strand, within GFF219 at 218.158 kb on - strand at 218.196 kb on + strand at 218.227 kb on + strand at 218.360 kb on + strand, within GFF220 at 218.360 kb on + strand, within GFF220 at 218.404 kb on + strand, within GFF220 at 218.405 kb on - strand, within GFF220 at 218.432 kb on - strand, within GFF220 at 218.439 kb on + strand, within GFF220 at 218.446 kb on - strand, within GFF220 at 218.497 kb on - strand, within GFF220 at 218.889 kb on + strand, within GFF220 at 218.918 kb on + strand, within GFF220 at 218.919 kb on - strand, within GFF220 at 218.948 kb on - strand, within GFF220 at 218.974 kb on + strand, within GFF220 at 218.975 kb on - strand, within GFF220 at 219.072 kb on + strand at 219.073 kb on - strand at 219.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 215,855 + GFF217 0.66 -0.4 215,940 - GFF217 0.76 -1.8 215,979 - GFF217 0.80 -0.8 216,009 - GFF217 0.83 +0.3 216,023 + GFF217 0.85 -1.4 216,095 + -0.6 216,132 - -0.2 216,250 + GFF218 0.21 -0.9 216,300 + GFF218 0.30 -0.2 216,300 + GFF218 0.30 -1.3 216,310 - GFF218 0.32 -0.2 216,634 + +0.2 216,634 + -0.5 216,634 + -0.8 216,634 + -0.9 216,634 + +0.2 216,996 - GFF219 0.20 -0.8 216,999 - GFF219 0.20 -0.9 217,528 - GFF219 0.55 +0.0 217,528 - GFF219 0.55 -0.6 217,633 + GFF219 0.62 +0.4 217,890 - GFF219 0.79 -0.2 217,923 + GFF219 0.81 -0.3 218,158 - +0.4 218,196 + -0.4 218,227 + -0.8 218,360 + GFF220 0.16 -0.9 218,360 + GFF220 0.16 +0.3 218,404 + GFF220 0.21 -0.6 218,405 - GFF220 0.21 -0.5 218,432 - GFF220 0.24 -0.9 218,439 + GFF220 0.25 +0.0 218,446 - GFF220 0.26 +0.3 218,497 - GFF220 0.32 -0.8 218,889 + GFF220 0.77 -0.3 218,918 + GFF220 0.80 +0.1 218,919 - GFF220 0.80 +3.0 218,948 - GFF220 0.84 -0.0 218,974 + GFF220 0.87 +0.6 218,975 - GFF220 0.87 +0.2 219,072 + -0.4 219,073 - -0.5 219,073 - +0.4
Or see this region's nucleotide sequence