Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2176 and GFF2177 are separated by 186 nucleotides GFF2177 and GFF2178 are separated by 46 nucleotides GFF2178 and GFF2179 overlap by 1 nucleotides GFF2179 and GFF2180 are separated by 145 nucleotides
GFF2176 - Putative permease PerM (= YfgO), at 23,630 to 24,688
GFF2176
GFF2177 - Glycerate kinase (EC 2.7.1.31), at 24,875 to 25,228
GFF2177
GFF2178 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 25,275 to 25,745
GFF2178
GFF2179 - Glycine cleavage system transcriptional antiactivator GcvR, at 25,745 to 26,317
GFF2179
GFF2180 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 26,463 to 27,341
GFF2180
Position (kb) 25
26 Strain fitness (log2 ratio) -1
0
1 at 24.332 kb on + strand, within GFF2176 at 24.332 kb on + strand, within GFF2176 at 24.381 kb on - strand, within GFF2176 at 24.728 kb on - strand at 25.155 kb on + strand, within GFF2177 at 25.399 kb on + strand, within GFF2178 at 25.400 kb on - strand, within GFF2178 at 25.750 kb on + strand at 25.779 kb on + strand at 25.793 kb on + strand at 25.795 kb on + strand at 25.796 kb on - strand at 25.820 kb on - strand, within GFF2179 at 25.820 kb on - strand, within GFF2179 at 25.820 kb on - strand, within GFF2179 at 25.824 kb on - strand, within GFF2179 at 25.967 kb on - strand, within GFF2179 at 26.165 kb on - strand, within GFF2179 at 26.248 kb on + strand, within GFF2179 at 26.249 kb on - strand, within GFF2179 at 26.295 kb on + strand at 26.295 kb on + strand at 26.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 24,332 + GFF2176 0.66 +0.8 24,332 + GFF2176 0.66 -0.5 24,381 - GFF2176 0.71 +0.2 24,728 - +0.1 25,155 + GFF2177 0.79 +1.2 25,399 + GFF2178 0.26 -0.7 25,400 - GFF2178 0.27 +0.2 25,750 + -0.5 25,779 + +0.0 25,793 + +0.0 25,795 + +0.0 25,796 - -0.3 25,820 - GFF2179 0.13 -0.0 25,820 - GFF2179 0.13 +0.0 25,820 - GFF2179 0.13 +0.2 25,824 - GFF2179 0.14 -0.3 25,967 - GFF2179 0.39 +0.1 26,165 - GFF2179 0.73 -0.0 26,248 + GFF2179 0.88 +0.2 26,249 - GFF2179 0.88 -0.1 26,295 + +0.2 26,295 + +0.5 26,296 - +0.3
Or see this region's nucleotide sequence