Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF216 and GFF217 are separated by 179 nucleotides GFF217 and GFF218 overlap by 23 nucleotides GFF218 and GFF219 are separated by 17 nucleotides
GFF216 - 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8), at 214,252 to 215,073
GFF216
GFF217 - '2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme- A synthase (EC 2.7.8.25)' transl_table=11, at 215,253 to 216,161
GFF217
GFF218 - Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.61), at 216,139 to 216,672
GFF218
GFF219 - Citrate lyase alpha chain (EC 4.1.3.6), at 216,690 to 218,210
GFF219
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1 at 215.192 kb on + strand at 215.334 kb on + strand at 215.339 kb on + strand at 215.397 kb on + strand, within GFF217 at 215.441 kb on - strand, within GFF217 at 215.441 kb on - strand, within GFF217 at 215.443 kb on + strand, within GFF217 at 215.443 kb on + strand, within GFF217 at 215.480 kb on + strand, within GFF217 at 215.855 kb on + strand, within GFF217 at 215.940 kb on - strand, within GFF217 at 215.979 kb on - strand, within GFF217 at 216.009 kb on - strand, within GFF217 at 216.023 kb on + strand, within GFF217 at 216.095 kb on + strand at 216.132 kb on - strand at 216.250 kb on + strand, within GFF218 at 216.300 kb on + strand, within GFF218 at 216.300 kb on + strand, within GFF218 at 216.310 kb on - strand, within GFF218 at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.634 kb on + strand at 216.996 kb on - strand, within GFF219 at 216.999 kb on - strand, within GFF219
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 215,192 + -0.1 215,334 + -0.7 215,339 + -2.1 215,397 + GFF217 0.16 -0.3 215,441 - GFF217 0.21 +0.1 215,441 - GFF217 0.21 -0.2 215,443 + GFF217 0.21 -0.1 215,443 + GFF217 0.21 -0.7 215,480 + GFF217 0.25 -0.4 215,855 + GFF217 0.66 -0.4 215,940 - GFF217 0.76 -1.8 215,979 - GFF217 0.80 -0.8 216,009 - GFF217 0.83 +0.3 216,023 + GFF217 0.85 -1.4 216,095 + -0.6 216,132 - -0.2 216,250 + GFF218 0.21 -0.9 216,300 + GFF218 0.30 -0.2 216,300 + GFF218 0.30 -1.3 216,310 - GFF218 0.32 -0.2 216,634 + +0.2 216,634 + -0.5 216,634 + -0.8 216,634 + -0.9 216,634 + +0.2 216,996 - GFF219 0.20 -0.8 216,999 - GFF219 0.20 -0.9
Or see this region's nucleotide sequence