Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2101 and GFF2102 are separated by 61 nucleotides GFF2102 and GFF2103 are separated by 102 nucleotides GFF2103 and GFF2104 are separated by 17 nucleotides
GFF2101 - Pyruvate formate-lyase (EC 2.3.1.54), at 10,395 to 10,778
GFF2101
GFF2102 - Transporter, LysE family, at 10,840 to 11,427
GFF2102
GFF2103 - LysR family transcriptional regulator YfiE, at 11,530 to 12,429
GFF2103
GFF2104 - ATP-dependent RNA helicase SrmB, at 12,447 to 13,781
GFF2104
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-1
0
1 at 10.890 kb on + strand at 10.894 kb on + strand at 10.987 kb on + strand, within GFF2102 at 10.987 kb on + strand, within GFF2102 at 10.987 kb on + strand, within GFF2102 at 10.988 kb on - strand, within GFF2102 at 10.990 kb on - strand, within GFF2102 at 11.040 kb on + strand, within GFF2102 at 11.040 kb on + strand, within GFF2102 at 11.183 kb on + strand, within GFF2102 at 11.183 kb on + strand, within GFF2102 at 11.391 kb on + strand at 11.392 kb on - strand at 11.392 kb on - strand at 11.392 kb on - strand at 11.392 kb on - strand at 11.411 kb on - strand at 11.564 kb on + strand at 11.914 kb on + strand, within GFF2103 at 11.973 kb on - strand, within GFF2103 at 12.079 kb on - strand, within GFF2103 at 12.190 kb on - strand, within GFF2103 at 12.201 kb on + strand, within GFF2103 at 12.240 kb on - strand, within GFF2103 at 12.267 kb on + strand, within GFF2103 at 12.371 kb on + strand at 12.375 kb on + strand at 12.375 kb on + strand at 12.375 kb on + strand at 12.376 kb on - strand at 12.442 kb on + strand at 12.442 kb on + strand at 12.669 kb on + strand, within GFF2104 at 12.909 kb on - strand, within GFF2104 at 12.912 kb on - strand, within GFF2104
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 10,890 + +0.4 10,894 + -1.1 10,987 + GFF2102 0.25 -0.5 10,987 + GFF2102 0.25 -0.2 10,987 + GFF2102 0.25 +0.3 10,988 - GFF2102 0.25 +0.4 10,990 - GFF2102 0.26 +0.1 11,040 + GFF2102 0.34 -0.3 11,040 + GFF2102 0.34 -0.1 11,183 + GFF2102 0.58 +0.5 11,183 + GFF2102 0.58 +0.9 11,391 + -0.9 11,392 - -0.8 11,392 - +0.2 11,392 - -1.0 11,392 - -0.4 11,411 - -0.8 11,564 + -0.2 11,914 + GFF2103 0.43 -0.3 11,973 - GFF2103 0.49 -1.5 12,079 - GFF2103 0.61 +0.1 12,190 - GFF2103 0.73 -1.1 12,201 + GFF2103 0.75 +0.2 12,240 - GFF2103 0.79 -1.0 12,267 + GFF2103 0.82 -0.4 12,371 + -0.3 12,375 + +0.2 12,375 + +0.8 12,375 + +0.8 12,376 - -0.7 12,442 + +0.1 12,442 + -0.1 12,669 + GFF2104 0.17 +0.1 12,909 - GFF2104 0.35 +0.4 12,912 - GFF2104 0.35 +0.1
Or see this region's nucleotide sequence