Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1844 and GFF1845 are separated by 272 nucleotides GFF1845 and GFF1846 overlap by 11 nucleotides
GFF1844 - Glycerol-3-phosphate transporter, at 34,998 to 36,356
GFF1844
GFF1845 - Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC 1.1.5.3), at 36,629 to 38,257
GFF1845
GFF1846 - Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC 1.1.5.3), at 38,247 to 39,506
GFF1846
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -1
0
1
2 at 35.784 kb on - strand, within GFF1844 at 35.786 kb on - strand, within GFF1844 at 36.130 kb on + strand, within GFF1844 at 36.131 kb on - strand, within GFF1844 at 36.189 kb on + strand, within GFF1844 at 36.189 kb on + strand, within GFF1844 at 36.347 kb on + strand at 36.357 kb on - strand at 36.451 kb on - strand at 36.451 kb on - strand at 36.456 kb on + strand at 36.456 kb on + strand at 36.476 kb on + strand at 36.486 kb on - strand at 36.582 kb on - strand at 36.752 kb on - strand at 36.752 kb on - strand at 36.753 kb on + strand at 36.907 kb on + strand, within GFF1845 at 36.910 kb on + strand, within GFF1845 at 37.451 kb on + strand, within GFF1845 at 37.673 kb on - strand, within GFF1845 at 38.154 kb on + strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.267 kb on + strand at 38.472 kb on + strand, within GFF1846 at 38.502 kb on - strand, within GFF1846 at 38.531 kb on + strand, within GFF1846 at 38.619 kb on - strand, within GFF1846 at 38.779 kb on + strand, within GFF1846 at 38.860 kb on - strand, within GFF1846 at 38.878 kb on + strand, within GFF1846 at 39.170 kb on - strand, within GFF1846
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 35,784 - GFF1844 0.58 +1.2 35,786 - GFF1844 0.58 +2.3 36,130 + GFF1844 0.83 +0.2 36,131 - GFF1844 0.83 -0.5 36,189 + GFF1844 0.88 -0.0 36,189 + GFF1844 0.88 +1.6 36,347 + +0.1 36,357 - +0.4 36,451 - -0.1 36,451 - -0.1 36,456 + +0.6 36,456 + -0.3 36,476 + -0.3 36,486 - -0.0 36,582 - -0.2 36,752 - -0.1 36,752 - +1.1 36,753 + +0.1 36,907 + GFF1845 0.17 -1.1 36,910 + GFF1845 0.17 +0.4 37,451 + GFF1845 0.50 -0.9 37,673 - GFF1845 0.64 -0.3 38,154 + -0.2 38,155 - +0.2 38,155 - -0.5 38,155 - +0.1 38,155 - -0.0 38,267 + -0.4 38,472 + GFF1846 0.18 +0.5 38,502 - GFF1846 0.20 +1.0 38,531 + GFF1846 0.23 +0.9 38,619 - GFF1846 0.30 -1.0 38,779 + GFF1846 0.42 +0.1 38,860 - GFF1846 0.49 -0.6 38,878 + GFF1846 0.50 -0.0 39,170 - GFF1846 0.73 +0.1
Or see this region's nucleotide sequence