Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1783 and GFF1784 overlap by 45 nucleotides GFF1784 and GFF1785 are separated by 125 nucleotides
GFF1783 - FIG00509904: hypothetical protein, at 5,396 to 6,685
GFF1783
GFF1784 - Dethiobiotin synthetase (EC 6.3.3.3), at 6,641 to 7,336
GFF1784
GFF1785 - Mlc, transcriptional repressor of MalT (the transcriptional activator of maltose regulon) and manXYZ operon, at 7,462 to 8,682
GFF1785
Position (kb) 6
7
8 Strain fitness (log2 ratio) -1
0
1 at 5.685 kb on - strand, within GFF1783 at 5.687 kb on - strand, within GFF1783 at 5.687 kb on - strand, within GFF1783 at 5.868 kb on + strand, within GFF1783 at 6.027 kb on + strand, within GFF1783 at 6.028 kb on - strand, within GFF1783 at 6.054 kb on - strand, within GFF1783 at 6.054 kb on - strand, within GFF1783 at 6.180 kb on + strand, within GFF1783 at 6.181 kb on - strand, within GFF1783 at 6.181 kb on - strand, within GFF1783 at 6.278 kb on - strand, within GFF1783 at 6.397 kb on + strand, within GFF1783 at 6.409 kb on - strand, within GFF1783 at 6.615 kb on + strand at 6.655 kb on + strand at 6.655 kb on + strand at 6.660 kb on - strand at 6.738 kb on - strand, within GFF1784 at 7.131 kb on + strand, within GFF1784 at 7.226 kb on - strand, within GFF1784 at 7.227 kb on + strand, within GFF1784 at 7.227 kb on + strand, within GFF1784 at 7.265 kb on + strand, within GFF1784 at 7.269 kb on - strand at 7.316 kb on - strand at 7.316 kb on - strand at 7.366 kb on - strand at 7.475 kb on - strand at 7.499 kb on + strand at 7.741 kb on - strand, within GFF1785 at 7.753 kb on + strand, within GFF1785 at 7.902 kb on + strand, within GFF1785 at 7.965 kb on + strand, within GFF1785 at 7.966 kb on - strand, within GFF1785 at 8.017 kb on - strand, within GFF1785 at 8.097 kb on + strand, within GFF1785 at 8.097 kb on + strand, within GFF1785 at 8.098 kb on - strand, within GFF1785 at 8.174 kb on + strand, within GFF1785
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 5,685 - GFF1783 0.22 +0.4 5,687 - GFF1783 0.23 +0.1 5,687 - GFF1783 0.23 +1.2 5,868 + GFF1783 0.37 +0.2 6,027 + GFF1783 0.49 -0.3 6,028 - GFF1783 0.49 -1.2 6,054 - GFF1783 0.51 -0.1 6,054 - GFF1783 0.51 -1.7 6,180 + GFF1783 0.61 -0.4 6,181 - GFF1783 0.61 -0.3 6,181 - GFF1783 0.61 +0.9 6,278 - GFF1783 0.68 +0.1 6,397 + GFF1783 0.78 +0.6 6,409 - GFF1783 0.79 +0.3 6,615 + +0.2 6,655 + +0.6 6,655 + +0.5 6,660 - -0.3 6,738 - GFF1784 0.14 -0.6 7,131 + GFF1784 0.70 -0.3 7,226 - GFF1784 0.84 -0.4 7,227 + GFF1784 0.84 -0.5 7,227 + GFF1784 0.84 -0.5 7,265 + GFF1784 0.90 +0.9 7,269 - -0.3 7,316 - -0.1 7,316 - -0.7 7,366 - +0.5 7,475 - -0.2 7,499 + +0.3 7,741 - GFF1785 0.23 -0.3 7,753 + GFF1785 0.24 -0.6 7,902 + GFF1785 0.36 +0.5 7,965 + GFF1785 0.41 -1.5 7,966 - GFF1785 0.41 +0.5 8,017 - GFF1785 0.45 +0.9 8,097 + GFF1785 0.52 +0.0 8,097 + GFF1785 0.52 -1.1 8,098 - GFF1785 0.52 +1.0 8,174 + GFF1785 0.58 -0.3
Or see this region's nucleotide sequence