Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1779 and GFF1780 are separated by 28 nucleotides GFF1780 and GFF1781 are separated by 9 nucleotides GFF1781 and GFF1782 overlap by 1 nucleotides
GFF1779 - Putative ABC transporter membrane protein, at 1,814 to 2,524
GFF1779
GFF1780 - Putative ABC transporter periplasmic binding protein, at 2,553 to 3,455
GFF1780
GFF1781 - Putative binding-protein-dependent transport system, at 3,465 to 4,112
GFF1781
GFF1782 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 4,112 to 5,260
GFF1782
Position (kb) 3
4
5 Strain fitness (log2 ratio) -2
-1
0
1 at 2.531 kb on - strand at 2.534 kb on + strand at 2.550 kb on + strand at 2.765 kb on + strand, within GFF1780 at 2.790 kb on + strand, within GFF1780 at 2.922 kb on - strand, within GFF1780 at 2.922 kb on - strand, within GFF1780 at 2.926 kb on - strand, within GFF1780 at 3.153 kb on + strand, within GFF1780 at 3.153 kb on + strand, within GFF1780 at 3.475 kb on + strand at 3.476 kb on - strand at 3.480 kb on + strand at 3.480 kb on + strand at 3.481 kb on - strand at 3.483 kb on - strand at 3.483 kb on - strand at 3.677 kb on - strand, within GFF1781 at 3.774 kb on - strand, within GFF1781 at 3.791 kb on + strand, within GFF1781 at 3.791 kb on + strand, within GFF1781 at 4.257 kb on + strand, within GFF1782 at 4.354 kb on + strand, within GFF1782 at 4.355 kb on - strand, within GFF1782 at 4.555 kb on + strand, within GFF1782 at 4.667 kb on - strand, within GFF1782 at 5.105 kb on - strand, within GFF1782
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 2,531 - +0.0 2,534 + +0.1 2,550 + +1.6 2,765 + GFF1780 0.23 +0.5 2,790 + GFF1780 0.26 +0.9 2,922 - GFF1780 0.41 -0.5 2,922 - GFF1780 0.41 +0.1 2,926 - GFF1780 0.41 -0.2 3,153 + GFF1780 0.66 -0.1 3,153 + GFF1780 0.66 -1.8 3,475 + +0.1 3,476 - +1.3 3,480 + +0.5 3,480 + +0.2 3,481 - -0.3 3,483 - -0.7 3,483 - +0.5 3,677 - GFF1781 0.33 -1.0 3,774 - GFF1781 0.48 -0.8 3,791 + GFF1781 0.50 -1.0 3,791 + GFF1781 0.50 -0.4 4,257 + GFF1782 0.13 +0.0 4,354 + GFF1782 0.21 +0.4 4,355 - GFF1782 0.21 -0.9 4,555 + GFF1782 0.39 -0.4 4,667 - GFF1782 0.48 +0.2 5,105 - GFF1782 0.86 -0.3
Or see this region's nucleotide sequence