Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1713 and GFF1714 are separated by 150 nucleotides GFF1714 and GFF1715 are separated by 89 nucleotides GFF1715 and GFF1716 are separated by 378 nucleotides
GFF1713 - Aspartate--ammonia ligase (EC 6.3.1.1), at 13,539 to 14,531
GFF1713
GFF1714 - Regulatory protein AsnC, at 14,682 to 15,140
GFF1714
GFF1715 - Flavoprotein MioC, at 15,230 to 15,673
GFF1715
GFF1716 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 16,052 to 17,941
GFF1716
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 13.879 kb on + strand, within GFF1713 at 13.879 kb on + strand, within GFF1713 at 13.880 kb on - strand, within GFF1713 at 14.076 kb on - strand, within GFF1713 at 14.099 kb on + strand, within GFF1713 at 14.114 kb on + strand, within GFF1713 at 14.195 kb on - strand, within GFF1713 at 14.254 kb on + strand, within GFF1713 at 14.393 kb on + strand, within GFF1713 at 14.393 kb on + strand, within GFF1713 at 14.403 kb on - strand, within GFF1713 at 14.563 kb on + strand at 14.877 kb on + strand, within GFF1714 at 14.980 kb on - strand, within GFF1714 at 15.005 kb on + strand, within GFF1714 at 15.047 kb on - strand, within GFF1714 at 15.197 kb on - strand at 15.241 kb on + strand at 15.241 kb on + strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.351 kb on + strand, within GFF1715 at 15.477 kb on + strand, within GFF1715 at 15.513 kb on - strand, within GFF1715 at 15.513 kb on - strand, within GFF1715 at 15.993 kb on + strand at 16.048 kb on + strand at 16.132 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 13,879 + GFF1713 0.34 +0.3 13,879 + GFF1713 0.34 -0.0 13,880 - GFF1713 0.34 -1.2 14,076 - GFF1713 0.54 +0.2 14,099 + GFF1713 0.56 -0.0 14,114 + GFF1713 0.58 -0.8 14,195 - GFF1713 0.66 +0.4 14,254 + GFF1713 0.72 -1.3 14,393 + GFF1713 0.86 +0.0 14,393 + GFF1713 0.86 +0.7 14,403 - GFF1713 0.87 -0.0 14,563 + -0.3 14,877 + GFF1714 0.42 -1.8 14,980 - GFF1714 0.65 -0.3 15,005 + GFF1714 0.70 +0.1 15,047 - GFF1714 0.80 -1.0 15,197 - -0.5 15,241 + -0.0 15,241 + -0.8 15,242 - +0.1 15,242 - +0.8 15,351 + GFF1715 0.27 -0.2 15,477 + GFF1715 0.56 -0.7 15,513 - GFF1715 0.64 -0.2 15,513 - GFF1715 0.64 +0.5 15,993 + +0.2 16,048 + -0.1 16,132 + -2.1
Or see this region's nucleotide sequence