Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1262 and GFF1263 are separated by 144 nucleotides GFF1263 and GFF1264 overlap by 1 nucleotides GFF1264 and GFF1265 are separated by 55 nucleotides GFF1265 and GFF1266 are separated by 71 nucleotides
GFF1262 - FIG01047494: hypothetical protein, at 89,370 to 89,558
GFF1262
GFF1263 - Adenine-specific methyltransferase (EC 2.1.1.72), at 89,703 to 90,383
GFF1263
GFF1264 - putative cytoplasmic protein, at 90,383 to 90,604
GFF1264
GFF1265 - FIG01046678: hypothetical protein, at 90,660 to 91,073
GFF1265
GFF1266 - FIG00638373: hypothetical protein, at 91,145 to 91,909
GFF1266
Position (kb) 90
91 Strain fitness (log2 ratio) -2
-1
0
1 at 89.438 kb on + strand, within GFF1262 at 89.503 kb on - strand, within GFF1262 at 89.702 kb on + strand at 89.702 kb on + strand at 89.703 kb on - strand at 89.738 kb on + strand at 89.738 kb on + strand at 89.739 kb on - strand at 89.791 kb on - strand, within GFF1263 at 89.825 kb on + strand, within GFF1263 at 89.884 kb on + strand, within GFF1263 at 89.916 kb on + strand, within GFF1263 at 89.989 kb on + strand, within GFF1263 at 89.990 kb on - strand, within GFF1263 at 90.004 kb on - strand, within GFF1263 at 90.114 kb on + strand, within GFF1263 at 90.115 kb on - strand, within GFF1263 at 90.267 kb on - strand, within GFF1263 at 90.563 kb on + strand, within GFF1264 at 90.564 kb on - strand, within GFF1264 at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.667 kb on - strand at 90.758 kb on + strand, within GFF1265 at 90.758 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.998 kb on - strand, within GFF1265 at 90.998 kb on - strand, within GFF1265 at 91.105 kb on + strand at 91.162 kb on + strand at 91.171 kb on - strand at 91.186 kb on + strand at 91.331 kb on - strand, within GFF1266 at 91.331 kb on - strand, within GFF1266 at 91.562 kb on - strand, within GFF1266
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 89,438 + GFF1262 0.36 +0.0 89,503 - GFF1262 0.70 +0.0 89,702 + +0.1 89,702 + -0.1 89,703 - -0.1 89,738 + -0.1 89,738 + -1.8 89,739 - +0.2 89,791 - GFF1263 0.13 -0.2 89,825 + GFF1263 0.18 -0.4 89,884 + GFF1263 0.27 -0.2 89,916 + GFF1263 0.31 -0.2 89,989 + GFF1263 0.42 -1.5 89,990 - GFF1263 0.42 -1.5 90,004 - GFF1263 0.44 +0.5 90,114 + GFF1263 0.60 +0.1 90,115 - GFF1263 0.60 +0.7 90,267 - GFF1263 0.83 +1.3 90,563 + GFF1264 0.81 -1.5 90,564 - GFF1264 0.82 -0.2 90,666 + -0.4 90,666 + -0.6 90,666 + +0.2 90,666 + -0.2 90,667 - +0.2 90,758 + GFF1265 0.24 +0.4 90,758 + GFF1265 0.24 +0.5 90,997 + GFF1265 0.81 -0.7 90,997 + GFF1265 0.81 -0.5 90,997 + GFF1265 0.81 -0.2 90,997 + GFF1265 0.81 +0.3 90,998 - GFF1265 0.82 +0.1 90,998 - GFF1265 0.82 +0.7 91,105 + -0.1 91,162 + +0.1 91,171 - +0.3 91,186 + +0.1 91,331 - GFF1266 0.24 -0.4 91,331 - GFF1266 0.24 +1.2 91,562 - GFF1266 0.55 -1.5
Or see this region's nucleotide sequence