Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1193 and GFF1194 are separated by 752 nucleotides GFF1194 and GFF1195 are separated by 581 nucleotides GFF1195 and GFF1196 are separated by 56 nucleotides GFF1196 and GFF1197 are separated by 48 nucleotides
GFF1193 - Endonuclease (EC 3.1.-.-), at 41,199 to 41,378
GFF1193
GFF1194 - DNA replication protein, at 42,131 to 43,012
GFF1194
GFF1195 - hypothetical protein, at 43,594 to 43,716
GFF1195
GFF1196 - hypothetical protein, at 43,773 to 43,904
GFF1196
GFF1197 - Outer membrane protein assembly factor YaeT precursor, at 43,953 to 44,093
GFF1197
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 41.248 kb on - strand, within GFF1193 at 41.309 kb on + strand, within GFF1193 at 41.313 kb on + strand, within GFF1193 at 41.313 kb on + strand, within GFF1193 at 41.376 kb on + strand at 41.387 kb on + strand at 41.388 kb on - strand at 41.441 kb on + strand at 41.441 kb on + strand at 41.442 kb on - strand at 41.495 kb on - strand at 41.507 kb on + strand at 41.534 kb on + strand at 41.600 kb on + strand at 41.642 kb on + strand at 41.642 kb on + strand at 41.727 kb on + strand at 41.727 kb on + strand at 41.789 kb on + strand at 41.792 kb on + strand at 41.792 kb on + strand at 41.873 kb on + strand at 42.028 kb on + strand at 42.029 kb on - strand at 42.072 kb on + strand at 42.072 kb on + strand at 42.128 kb on + strand at 42.245 kb on - strand, within GFF1194 at 42.245 kb on - strand, within GFF1194 at 42.445 kb on - strand, within GFF1194 at 42.518 kb on - strand, within GFF1194 at 42.538 kb on + strand, within GFF1194 at 42.590 kb on - strand, within GFF1194 at 42.771 kb on - strand, within GFF1194 at 43.009 kb on - strand at 43.010 kb on + strand at 43.011 kb on - strand at 43.115 kb on + strand at 43.334 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.429 kb on - strand at 43.432 kb on - strand at 43.661 kb on + strand, within GFF1195 at 43.661 kb on + strand, within GFF1195 at 43.662 kb on - strand, within GFF1195 at 43.662 kb on - strand, within GFF1195 at 43.787 kb on + strand, within GFF1196 at 43.787 kb on + strand, within GFF1196 at 43.787 kb on + strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.842 kb on - strand, within GFF1196 at 43.853 kb on - strand, within GFF1196 at 43.887 kb on + strand, within GFF1196
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 41,248 - GFF1193 0.27 +0.4 41,309 + GFF1193 0.61 +0.8 41,313 + GFF1193 0.63 +0.5 41,313 + GFF1193 0.63 +1.2 41,376 + +2.2 41,387 + +0.1 41,388 - +0.2 41,441 + +0.1 41,441 + +0.1 41,442 - +0.4 41,495 - -0.7 41,507 + -0.4 41,534 + +0.8 41,600 + +1.1 41,642 + +0.6 41,642 + +0.7 41,727 + +0.4 41,727 + +0.6 41,789 + +0.9 41,792 + +0.6 41,792 + +0.8 41,873 + +1.1 42,028 + -0.2 42,029 - +0.8 42,072 + -0.1 42,072 + -0.1 42,128 + +0.3 42,245 - GFF1194 0.13 -1.2 42,245 - GFF1194 0.13 -0.8 42,445 - GFF1194 0.36 -0.8 42,518 - GFF1194 0.44 -0.4 42,538 + GFF1194 0.46 +0.3 42,590 - GFF1194 0.52 -0.7 42,771 - GFF1194 0.73 -0.7 43,009 - -0.5 43,010 + -1.8 43,011 - +0.8 43,115 + -0.6 43,334 + +0.4 43,428 + +0.7 43,428 + -0.2 43,428 + +0.4 43,428 + +0.1 43,429 - +1.8 43,432 - +0.3 43,661 + GFF1195 0.54 -0.4 43,661 + GFF1195 0.54 -0.1 43,662 - GFF1195 0.55 -0.7 43,662 - GFF1195 0.55 -0.1 43,787 + GFF1196 0.11 +0.3 43,787 + GFF1196 0.11 -0.2 43,787 + GFF1196 0.11 -0.2 43,788 - GFF1196 0.11 +0.9 43,788 - GFF1196 0.11 +0.2 43,788 - GFF1196 0.11 +0.4 43,817 - GFF1196 0.33 +1.6 43,817 - GFF1196 0.33 -0.1 43,817 - GFF1196 0.33 -0.4 43,842 - GFF1196 0.52 -1.0 43,853 - GFF1196 0.61 +2.8 43,887 + GFF1196 0.86 -1.6
Or see this region's nucleotide sequence