Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1162 and GFF1163 are separated by 134 nucleotides GFF1163 and GFF1164 overlap by 8 nucleotides GFF1164 and GFF1165 overlap by 4 nucleotides GFF1165 and GFF1166 are separated by 0 nucleotides GFF1166 and GFF1167 are separated by 15 nucleotides
GFF1162 - IncF plasmid conjugative transfer pilus assembly protein TraV, at 13,150 to 13,665
GFF1162
GFF1163 - IncF plasmid conjugative transfer protein TraR, at 13,800 to 14,021
GFF1163
GFF1164 - Conjugative transfer protein PSLT087, at 14,014 to 14,481
GFF1164
GFF1165 - hypothetical protein, at 14,478 to 14,597
GFF1165
GFF1166 - FIG01045198: hypothetical protein, at 14,598 to 14,861
GFF1166
GFF1167 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 14,877 to 17,525
GFF1167
Position (kb) 14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 13.576 kb on + strand, within GFF1162 at 13.576 kb on + strand, within GFF1162 at 13.594 kb on - strand, within GFF1162 at 13.597 kb on + strand, within GFF1162 at 13.597 kb on + strand, within GFF1162 at 13.598 kb on - strand, within GFF1162 at 13.684 kb on - strand at 13.825 kb on - strand, within GFF1163 at 13.858 kb on + strand, within GFF1163 at 14.019 kb on + strand at 14.055 kb on + strand at 14.151 kb on - strand, within GFF1164 at 14.224 kb on - strand, within GFF1164 at 14.245 kb on + strand, within GFF1164 at 14.246 kb on - strand, within GFF1164 at 14.247 kb on + strand, within GFF1164 at 14.298 kb on + strand, within GFF1164 at 14.401 kb on - strand, within GFF1164 at 14.554 kb on - strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.565 kb on - strand, within GFF1165 at 14.605 kb on + strand at 14.761 kb on - strand, within GFF1166 at 15.024 kb on - strand at 15.068 kb on + strand at 15.204 kb on - strand, within GFF1167 at 15.204 kb on - strand, within GFF1167 at 15.204 kb on - strand, within GFF1167 at 15.276 kb on + strand, within GFF1167 at 15.276 kb on + strand, within GFF1167 at 15.279 kb on + strand, within GFF1167 at 15.279 kb on + strand, within GFF1167 at 15.280 kb on - strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.457 kb on - strand, within GFF1167 at 15.457 kb on - strand, within GFF1167 at 15.515 kb on + strand, within GFF1167 at 15.579 kb on + strand, within GFF1167 at 15.579 kb on + strand, within GFF1167
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 13,576 + GFF1162 0.83 -0.6 13,576 + GFF1162 0.83 -1.2 13,594 - GFF1162 0.86 +0.4 13,597 + GFF1162 0.87 -0.2 13,597 + GFF1162 0.87 +0.0 13,598 - GFF1162 0.87 -0.6 13,684 - -0.3 13,825 - GFF1163 0.11 +0.0 13,858 + GFF1163 0.26 -0.9 14,019 + -2.5 14,055 + +0.3 14,151 - GFF1164 0.29 +0.2 14,224 - GFF1164 0.45 +0.5 14,245 + GFF1164 0.49 +1.1 14,246 - GFF1164 0.50 +0.0 14,247 + GFF1164 0.50 -0.0 14,298 + GFF1164 0.61 -0.8 14,401 - GFF1164 0.83 -0.4 14,554 - GFF1165 0.63 -0.3 14,564 + GFF1165 0.72 -0.1 14,564 + GFF1165 0.72 -0.0 14,564 + GFF1165 0.72 -1.3 14,565 - GFF1165 0.72 -1.4 14,605 + -0.8 14,761 - GFF1166 0.62 +0.8 15,024 - +0.2 15,068 + +0.3 15,204 - GFF1167 0.12 -0.8 15,204 - GFF1167 0.12 -0.2 15,204 - GFF1167 0.12 -0.3 15,276 + GFF1167 0.15 -0.0 15,276 + GFF1167 0.15 +0.3 15,279 + GFF1167 0.15 +0.1 15,279 + GFF1167 0.15 +0.4 15,280 - GFF1167 0.15 -0.7 15,456 + GFF1167 0.22 -0.1 15,456 + GFF1167 0.22 -1.1 15,456 + GFF1167 0.22 -0.2 15,457 - GFF1167 0.22 +0.1 15,457 - GFF1167 0.22 +0.3 15,515 + GFF1167 0.24 -0.0 15,579 + GFF1167 0.27 -0.8 15,579 + GFF1167 0.27 -0.3
Or see this region's nucleotide sequence