Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1146 and GFF1147 are separated by 34 nucleotides GFF1147 and GFF1148 are separated by 73 nucleotides GFF1148 and GFF1149 are separated by 156 nucleotides GFF1149 and GFF1150 are separated by 103 nucleotides GFF1150 and GFF1151 are separated by 41 nucleotides GFF1151 and GFF1152 are separated by 310 nucleotides
GFF1146 - PsiA protein, at 4,037 to 4,774
GFF1146
GFF1147 - hypothetical protein, at 4,809 to 4,937
GFF1147
GFF1148 - FIG01049080: hypothetical protein, at 5,011 to 5,181
GFF1148
GFF1149 - unnamed protein product, at 5,338 to 5,553
GFF1149
GFF1150 - unnamed protein product, at 5,657 to 5,890
GFF1150
GFF1151 - FIG01047736: hypothetical protein, at 5,932 to 6,102
GFF1151
GFF1152 - X polypeptide, at 6,413 to 6,883
GFF1152
Position (kb) 5
6 Strain fitness (log2 ratio) -1
0
1
2 at 4.667 kb on + strand, within GFF1146 at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.990 kb on + strand at 5.039 kb on + strand, within GFF1148 at 5.111 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.267 kb on + strand at 5.269 kb on + strand at 5.271 kb on + strand at 5.421 kb on + strand, within GFF1149 at 5.504 kb on + strand, within GFF1149 at 5.529 kb on + strand, within GFF1149 at 5.618 kb on - strand at 5.667 kb on - strand at 5.689 kb on - strand, within GFF1150 at 5.942 kb on + strand at 5.943 kb on - strand at 6.109 kb on - strand at 6.143 kb on + strand at 6.179 kb on - strand at 6.236 kb on - strand at 6.604 kb on - strand, within GFF1152
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 4,667 + GFF1146 0.85 +0.4 4,703 + -0.1 4,703 + -1.3 4,703 + -0.0 4,703 + -0.8 4,703 + +0.3 4,703 + +0.0 4,704 - -0.7 4,704 - -0.2 4,704 - +0.1 4,704 - +0.5 4,889 + GFF1147 0.62 +0.1 4,889 + GFF1147 0.62 +0.1 4,889 + GFF1147 0.62 -0.3 4,889 + GFF1147 0.62 +2.1 4,889 + GFF1147 0.62 -0.3 4,890 - GFF1147 0.63 +0.5 4,890 - GFF1147 0.63 -0.4 4,890 - GFF1147 0.63 +0.1 4,990 + +0.1 5,039 + GFF1148 0.16 +1.0 5,111 - GFF1148 0.58 +0.2 5,130 - GFF1148 0.70 -0.3 5,130 - GFF1148 0.70 +0.2 5,130 - GFF1148 0.70 -0.2 5,267 + +0.9 5,269 + +0.1 5,271 + +0.7 5,421 + GFF1149 0.38 +1.4 5,504 + GFF1149 0.77 -0.4 5,529 + GFF1149 0.88 -0.7 5,618 - +0.2 5,667 - -0.3 5,689 - GFF1150 0.14 -0.6 5,942 + +0.3 5,943 - +0.3 6,109 - -0.1 6,143 + -0.2 6,179 - -0.3 6,236 - +0.1 6,604 - GFF1152 0.41 -0.4
Or see this region's nucleotide sequence