Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1051 and GFF1052 are separated by 70 nucleotides GFF1052 and GFF1053 are separated by 101 nucleotides GFF1053 and GFF1054 are separated by 33 nucleotides GFF1054 and GFF1055 overlap by 33 nucleotides GFF1055 and GFF1056 overlap by 1 nucleotides
GFF1051 - Beta-fimbriae probable major subunit, at 233,763 to 234,452
GFF1051
GFF1052 - hypothetical protein, at 234,523 to 234,663
GFF1052
GFF1053 - Lipoate-protein ligase A, at 234,765 to 235,781
GFF1053
GFF1054 - Lipoate-protein ligase A, at 235,815 to 236,459
GFF1054
GFF1055 - Phosphoserine phosphatase (EC 3.1.3.3), at 236,427 to 236,576
GFF1055
GFF1056 - Phosphoserine phosphatase (EC 3.1.3.3), at 236,576 to 237,544
GFF1056
Position (kb) 234
235
236 Strain fitness (log2 ratio) -1
0
1
2 at 233.770 kb on + strand at 233.772 kb on + strand at 233.781 kb on - strand at 233.841 kb on + strand, within GFF1051 at 233.854 kb on - strand, within GFF1051 at 233.880 kb on - strand, within GFF1051 at 233.913 kb on - strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.937 kb on - strand, within GFF1051 at 233.943 kb on - strand, within GFF1051 at 234.061 kb on + strand, within GFF1051 at 234.110 kb on - strand, within GFF1051 at 234.117 kb on + strand, within GFF1051 at 234.118 kb on - strand, within GFF1051 at 234.122 kb on + strand, within GFF1051 at 234.123 kb on - strand, within GFF1051 at 234.242 kb on + strand, within GFF1051 at 234.339 kb on - strand, within GFF1051 at 234.339 kb on - strand, within GFF1051 at 234.375 kb on + strand, within GFF1051 at 234.859 kb on - strand at 234.859 kb on - strand at 234.859 kb on - strand at 234.936 kb on - strand, within GFF1053 at 235.209 kb on + strand, within GFF1053 at 235.210 kb on - strand, within GFF1053 at 235.601 kb on + strand, within GFF1053 at 235.613 kb on + strand, within GFF1053 at 235.761 kb on - strand at 235.771 kb on - strand at 235.901 kb on + strand, within GFF1054 at 235.902 kb on - strand, within GFF1054 at 235.909 kb on - strand, within GFF1054 at 236.183 kb on - strand, within GFF1054 at 236.382 kb on - strand, within GFF1054 at 236.413 kb on - strand at 236.413 kb on - strand at 236.486 kb on + strand, within GFF1055 at 236.508 kb on + strand, within GFF1055 at 236.508 kb on + strand, within GFF1055 at 236.509 kb on - strand, within GFF1055 at 236.563 kb on + strand at 236.581 kb on + strand at 236.587 kb on + strand at 236.588 kb on - strand at 236.672 kb on + strand at 236.672 kb on + strand at 236.720 kb on - strand, within GFF1056 at 236.730 kb on + strand, within GFF1056 at 236.738 kb on + strand, within GFF1056
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 233,770 + -0.1 233,772 + -0.5 233,781 - -0.3 233,841 + GFF1051 0.11 -1.7 233,854 - GFF1051 0.13 -0.2 233,880 - GFF1051 0.17 +0.1 233,913 - GFF1051 0.22 +0.1 233,914 + GFF1051 0.22 +0.4 233,914 + GFF1051 0.22 -0.8 233,914 + GFF1051 0.22 -0.3 233,915 - GFF1051 0.22 -0.9 233,915 - GFF1051 0.22 +0.2 233,915 - GFF1051 0.22 +0.1 233,918 + GFF1051 0.22 +1.0 233,918 + GFF1051 0.22 -0.1 233,937 - GFF1051 0.25 +0.4 233,943 - GFF1051 0.26 -0.4 234,061 + GFF1051 0.43 -1.7 234,110 - GFF1051 0.50 +0.4 234,117 + GFF1051 0.51 -0.4 234,118 - GFF1051 0.51 +0.8 234,122 + GFF1051 0.52 -0.1 234,123 - GFF1051 0.52 -0.4 234,242 + GFF1051 0.69 +0.1 234,339 - GFF1051 0.83 +0.2 234,339 - GFF1051 0.83 +1.1 234,375 + GFF1051 0.89 -0.8 234,859 - -0.4 234,859 - -0.3 234,859 - +0.2 234,936 - GFF1053 0.17 +0.3 235,209 + GFF1053 0.44 -0.3 235,210 - GFF1053 0.44 +0.5 235,601 + GFF1053 0.82 +1.1 235,613 + GFF1053 0.83 +0.5 235,761 - +0.3 235,771 - +1.1 235,901 + GFF1054 0.13 +0.0 235,902 - GFF1054 0.13 -0.3 235,909 - GFF1054 0.15 -0.2 236,183 - GFF1054 0.57 -0.6 236,382 - GFF1054 0.88 +0.1 236,413 - -0.1 236,413 - +0.2 236,486 + GFF1055 0.39 +0.3 236,508 + GFF1055 0.54 +0.5 236,508 + GFF1055 0.54 -1.1 236,509 - GFF1055 0.55 -0.1 236,563 + +0.1 236,581 + +0.0 236,587 + +0.4 236,588 - -0.1 236,672 + -0.0 236,672 + -0.3 236,720 - GFF1056 0.15 +0.8 236,730 + GFF1056 0.16 -0.3 236,738 + GFF1056 0.17 +2.0
Or see this region's nucleotide sequence