Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF101 and GFF102 are separated by 108 nucleotides GFF102 and GFF103 are separated by 57 nucleotides GFF103 and GFF104 are separated by 205 nucleotides
GFF101 - ABC-type multidrug transport system, ATPase component, at 85,983 to 86,909
GFF101
GFF102 - Carbonic anhydrase (EC 4.2.1.1), at 87,018 to 87,680
GFF102
GFF103 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 87,738 to 88,274
GFF103
GFF104 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 88,480 to 90,870
GFF104
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-2
-1
0
1 at 86.760 kb on - strand, within GFF101 at 86.922 kb on - strand at 86.945 kb on + strand at 86.946 kb on - strand at 86.946 kb on - strand at 86.992 kb on + strand at 87.663 kb on + strand at 87.679 kb on - strand at 87.686 kb on + strand at 87.690 kb on + strand at 87.690 kb on + strand at 87.691 kb on - strand at 87.691 kb on - strand at 87.693 kb on - strand at 87.701 kb on + strand at 87.701 kb on + strand at 87.769 kb on + strand at 87.770 kb on - strand at 87.897 kb on - strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.972 kb on - strand, within GFF103 at 87.999 kb on - strand, within GFF103 at 88.137 kb on - strand, within GFF103 at 88.190 kb on - strand, within GFF103 at 88.223 kb on + strand at 88.322 kb on - strand at 88.325 kb on - strand at 88.355 kb on - strand at 88.432 kb on - strand at 88.434 kb on - strand at 88.479 kb on + strand at 88.512 kb on - strand at 88.650 kb on - strand at 88.793 kb on + strand, within GFF104
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 86,760 - GFF101 0.84 -0.2 86,922 - +0.1 86,945 + +0.5 86,946 - +0.4 86,946 - -0.2 86,992 + +0.0 87,663 + +0.1 87,679 - -1.1 87,686 + -0.5 87,690 + +0.9 87,690 + -0.9 87,691 - -2.2 87,691 - +1.6 87,693 - +0.4 87,701 + -0.8 87,701 + -0.7 87,769 + -0.9 87,770 - -0.9 87,897 - GFF103 0.30 -0.1 87,956 + GFF103 0.41 -1.4 87,956 + GFF103 0.41 -0.8 87,956 + GFF103 0.41 -0.9 87,972 - GFF103 0.44 -0.7 87,999 - GFF103 0.49 -0.1 88,137 - GFF103 0.74 +0.6 88,190 - GFF103 0.84 -1.4 88,223 + -0.4 88,322 - -0.7 88,325 - -0.2 88,355 - +0.3 88,432 - +0.5 88,434 - +0.5 88,479 + +0.6 88,512 - +0.9 88,650 - +0.6 88,793 + GFF104 0.13 -1.5
Or see this region's nucleotide sequence